Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

Nuclear Stability

Nuclear Stability

Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...

Stability

Stability

The time response of a linear time-invariant (LTI) system can be divided into transient and steady-state responses. The transient response represents the system's initial reaction to a change in input and diminishes to zero over time. In contrast, the steady-state response is the behavior that persists after the transient effects have faded.
The stability of an LTI system is determined by the roots of its characteristic equation, known as poles. A system is stable if it produces a bounded...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

B Cell Activation and Differentiation

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Taxonomic Diversity and Metabolic and Pharmacological Profiles of Marine-Derived Actinomycetes from the Lisbon and Setúbal Coast, Portugal.

Marine drugs·2026

Same author

Multifunctional Nanovaccine Sensitizes Breast Cancer to Immune Checkpoint Therapy.

Advanced functional materials·2025

Same author

Liposomal formulations of novel metal-based complexes exhibiting high antitumor activity against melanoma.

International journal of pharmaceutics·2025

Same author

Hybrid Molecules of p53 Activators and Protoflavones to Target Multiple Myeloma.

ChemMedChem·2025

Same author

Polymeric-based neoantigen nanovaccine synergizes with PD-1/PD-L1 modulators, reprogramming the melanoma microenvironment.

Journal of controlled release : official journal of the Controlled Release Society·2025

Same author

Role of CNNM4 in the progression of cholangiocarcinoma: implications for ferroptosis and therapeutic potential.

Gut·2025

Search research articles

Related Experiment Video

Updated: Jan 29, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

A Novel Small Molecule p53 Stabilizer for Brain Cell Differentiation.

Joana D Amaral¹, Dário Silva¹, Cecília M P Rodrigues¹

¹Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal.

Frontiers in Chemistry

|February 16, 2019

Summary

A novel spiropyrazoline oxindole compound selectively targets brain tumor stem cells, inducing differentiation and reducing cancer recurrence. This molecule shows promise as a non-toxic agent for developing new brain antitumor drugs.

Keywords:

antiproliferative agents brain tumor cell differentiation p53 spirooxindole

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Published on: May 19, 2023

Related Experiment Videos

Last Updated: Jan 29, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Published on: May 19, 2023

Area of Science:

Medicinal Chemistry
Cancer Biology
Neuro-oncology

Background:

Cancer stem cells drive tumor recurrence and therapy resistance.
Targeting stemness properties is crucial for effective cancer treatment.
Spiropyrazoline oxindoles show potential in modulating cancer cell pathways.

Purpose of the Study:

To synthesize novel spiropyrazoline oxindoles to modulate p53 activity and induce cancer stem cell differentiation.
To identify compounds that selectively target cancer cells and promote differentiation.
To evaluate the potential of these compounds as non-toxic differentiation inducers for brain tumors.

Main Methods:

Synthesis and chemical modification of spiropyrazoline oxindoles.
Antiproliferative activity assessment using isogenic HCT116 cell lines (p53 proficient and deficient).
Evaluation of stemness markers (SOX2, βIII-tubulin) in neural stem cells and glioma cells (GL-261).

Main Results:

Spiropyrazoline oxindole 1a demonstrated selective antiproliferative activity against p53-expressing cancer cells with low cytotoxicity.
Compound 1a induced neural stem cell differentiation, decreasing SOX2 and increasing βIII-tubulin.
In glioma cells, compound 1a reduced stemness (decreased SOX2) and enhanced chemotherapy sensitization.

Conclusions:

Novel spiropyrazoline oxindoles can modulate p53 activity and induce cell differentiation.
Compound 1a is a promising lead molecule for non-toxic brain tumor therapy by targeting cancer stemness.
Targeting p53 and stemness pathways offers a potential strategy for novel brain antitumor drug development.