Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

1.2K
Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
1.2K
Nuclear Stability03:18

Nuclear Stability

23.2K
Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...
23.2K
RNA Stability01:53

RNA Stability

35.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.7K
Stability01:28

Stability

414
The time response of a linear time-invariant (LTI) system can be divided into transient and steady-state responses. The transient response represents the system's initial reaction to a change in input and diminishes to zero over time. In contrast, the steady-state response is the behavior that persists after the transient effects have faded.
The stability of an LTI system is determined by the roots of its characteristic equation, known as poles. A system is stable if it produces a bounded...
414
Stability of structures01:14

Stability of structures

511
In mechanical engineering, the stability of systems under various forces is critical for designing durable and efficient structures. One fundamental way to explore these concepts is by analyzing systems like two rods connected at a pivot point, O, with a torsional spring of spring constant k at the pivot point. This system is similar in appearance to a scissor jack used to change tires on a car. In this case, the arms of the linkage (equivalent to the rods in this system) are entirely vertical,...
511
Pole and System Stability01:24

Pole and System Stability

950
The transfer function is a fundamental concept representing the ratio of two polynomials. The numerator and denominator encapsulate the system's dynamics. The zeros and poles of this transfer function are critical in determining the system's behavior and stability.
Simple poles are unique roots of the denominator polynomial. Each simple pole corresponds to a distinct solution to the system's characteristic equation, typically resulting in exponential decay terms in the system's...
950

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A reversible broad-spectrum antiviral targets the human V-ATPase VO domain.

Research square·2026
Same author

Global impact through train-the-trainer (TtT) courses.

Journal of microscopy·2026
Same author

Natufian sickle blades (ca. 15,000-11,700 cal. BP) reveal cereal cultivation ca. 4.5 millennia before domestication.

Scientific reports·2026
Same author

People-centred funding as a catalyst for sustainable imaging infrastructure.

Journal of microscopy·2026
Same author

Deuterated Pentoxifylline Analog in the Treatment of Necrobiosis Lipoidica: A Nonrandomized Clinical Trial.

JAMA dermatology·2026
Same author

Making the invisible visible: A global examination of careers and recognition for Imaging Scientists in core facilities.

Journal of microscopy·2026
Same journal

Altered expression of Toll-like receptor 9 in the lung tissue of adult mice generated by in vitro embryo culture and embryo transfer.

Histochemistry and cell biology·2026
Same journal

Dynamic changes in OTULIN and progranulin levels in experimental myocardial infarction and cardiac remodeling.

Histochemistry and cell biology·2026
Same journal

Eosinophil-associated matrix remodeling in a sterile granulomatous inflammation model: a temporal histopathological analysis.

Histochemistry and cell biology·2026
Same journal

Cellular accumulation of lipofuscin in the heart: implications in health and disease.

Histochemistry and cell biology·2026
Same journal

From lipofuscin accumulation to cellular dysfunction: a focus on liver pathophysiology.

Histochemistry and cell biology·2026
Same journal

Immunohistochemical study of α-keratin, loricrin, filaggrin-like protein, and transglutaminase-1 expression in orthokeratinized and parakeratinized epithelium of the tongue of domestic goose (Anser anser f. domestica) during embryonic development.

Histochemistry and cell biology·2026
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

Major Components of the Light Microscope
08:08

Major Components of the Light Microscope

Published on: July 30, 2008

24.5K

Fluorescence microscope light source stability.

Firas Mubaid1, Daniel Kaufman1, Tse-Luen Wee1,2

  • 1Department of Physiology, McGill University, Montreal, Canada.

Histochemistry and Cell Biology
|February 16, 2019
PubMed
Summary
This summary is machine-generated.

Light-emitting diodes (LEDs) offer superior stability and lower power consumption for fluorescence microscopy compared to traditional arc lamps. LEDs also provide environmental benefits, such as mercury-free operation and reduced lifecycle impact.

Keywords:
FluorescenceLEDLight sourceMicroscopySolid stateStability

More Related Videos

Plasmid Stability Analysis with Open-Source Droplet Microfluidics
07:43

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

1.1K
Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
10:32

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

Published on: April 23, 2021

3.2K

Related Experiment Videos

Last Updated: Jan 29, 2026

Major Components of the Light Microscope
08:08

Major Components of the Light Microscope

Published on: July 30, 2008

24.5K
Plasmid Stability Analysis with Open-Source Droplet Microfluidics
07:43

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

1.1K
Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
10:32

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

Published on: April 23, 2021

3.2K

Area of Science:

  • Microscopy
  • Optical Engineering
  • Environmental Science

Background:

  • Fluorescence microscopy relies on efficient light sources for fluorophore excitation.
  • Traditional arc lamps have been widely used, but newer technologies offer potential advantages.
  • Illumination choice impacts experimental quality, cost, and environmental footprint.

Purpose of the Study:

  • To compare the performance and environmental impact of light-emitting diodes (LEDs) with traditional light sources for fluorescence microscopy.
  • To evaluate the stability, power consumption, and operational characteristics of different illumination systems.
  • To provide protocols for testing light source stability.

Main Methods:

  • Comparative analysis of LED and traditional light sources (arc lamps, metal halide).
  • Measurement of light stability across various timescales and operational conditions (warm-up, switching).
  • Electrical power consumption assessment.
  • Lifecycle analysis for environmental impact evaluation.

Main Results:

  • LEDs exhibit superior light stability compared to traditional sources.
  • LEDs demonstrate lower electrical power usage, especially at lower outputs.
  • LEDs offer advantages like electronic switching, longer lifetimes, and mercury-free composition.
  • Lifecycle analysis indicates a fourfold lower environmental impact for LED sources versus metal halide.

Conclusions:

  • LEDs are a preferred light source for modern fluorescence microscopy due to enhanced stability, efficiency, and environmental benefits.
  • Careful measurement of light source power output during operation is crucial.
  • LED technology significantly reduces the environmental burden associated with fluorescence microscopy illumination.