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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Stomach Histology01:26

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The stomach comprises several layers that work together to facilitate digestion and protect the organ. The outermost layer is called the serosa, which provides support and protection to the stomach. The muscularis externa layer is responsible for the mechanical breakdown of food by contracting and moving the stomach. The submucosa layer, located beneath the muscularis externa, contains connective tissue, blood vessels, nerves, and glands that secrete mucus and other substances essential for...
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Liver Histology01:27

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The microscopic anatomy of the liver is a complex and intricate system that comprises numerous structural units known as liver lobules, each of which is comparable in size to a sesame seed. These hexagonal structures consist of plates of liver cells or hepatocytes, which are characterized by their versatility and abundance of cellular apparatus like rough and smooth ER, Golgi apparatus, peroxisomes, and mitochondria.
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Histology of the Large Intestine01:26

Histology of the Large Intestine

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The large intestine, a vital component of the gastrointestinal tract, is structured with four main layers: the mucosa, submucosa, muscularis, and serosa. Each layer performs a distinct role in facilitating the smooth functioning of the large intestine.
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Related Experiment Video

Updated: Jan 29, 2026

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
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Rapid histologic diagnosis using quick fluorescence staining and tissue confocal microscopy.

Hongrae Kim1, Sunhye Lee1, Hyunjin Kim2

  • 1Innovative Medical Engineering & Technology, National Cancer Center, Goyang, South Korea.

Microscopy Research and Technique
|February 16, 2019
PubMed
Summary

This study introduces a novel, rapid tissue diagnosis method using confocal microscopy and simple staining, eliminating the need for freezing and slicing. This technique aims to improve real-time surgical decision-making, enhancing patient outcomes.

Keywords:
confocal microscopyfluorescent imageminimally invasive surgeryquick staining methodtissue staining

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Area of Science:

  • Surgical Pathology
  • Microscopy Techniques
  • Biomedical Imaging

Background:

  • Minimally invasive surgery necessitates rapid, accurate intraoperative tissue diagnosis.
  • Current methods like frozen sections are time-consuming and require specialized equipment.
  • Confocal microscopy offers high-resolution imaging but lacks widespread intraoperative application.

Purpose of the Study:

  • To develop a simple, rapid tissue staining method for intraoperative confocal microscopy.
  • To eliminate the need for tissue freezing and slicing in diagnostic procedures.
  • To enable real-time tissue analysis during surgery.

Main Methods:

  • Development of a fluorescence staining protocol using Hoechst 33342 and Eosin.
  • Application of the staining method to tissue samples.
  • Intraoperative imaging using confocal microscopy.

Main Results:

  • The proposed staining method does not require tissue freezing or slicing.
  • Achieved rapid tissue visualization suitable for operating room conditions.
  • Demonstrated potential for real-time tissue diagnosis.

Conclusions:

  • A novel fluorescence staining technique using Hoechst 33342 and Eosin is proposed for intraoperative confocal microscopy.
  • This method offers a potential solution for rapid tissue diagnosis in surgical settings.
  • Further research is needed for clinical implementation.