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Detached-Leaf Method for Propagating Puccinia coronata and Assessing Crown Rust Resistance in Oat
E W Jackson1, D E Obert1, J Chong2
1United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Small Grains and Potato Germplasm Research Unit, Aberdeen, ID 83210.
Plant Disease
|February 17, 2019
Summary
This study presents a novel detached-leaf system for propagating the crown rust pathogen, Puccinia coronata. This method facilitates controlled evaluation of oat resistance to P. coronata races.
Area of Science:
- Plant Pathology
- Agricultural Science
- Mycology
Background:
- The crown rust pathogen, Puccinia coronata, is an obligate biotroph with wind-disseminated spores and numerous races.
- Propagation of single-race cultures and genetic studies are challenging due to contamination risks in field and greenhouse settings.
Purpose of the Study:
- To develop an isolated propagation system for Puccinia coronata.
- To test the system's efficacy in assessing oat (Avena sativa) host resistance.
Main Methods:
- Oat leaf sections were disinfested and supported by agar blocks in sterile boxes.
- Inoculated leaf sections were incubated in a lighted growth cabinet until sporulation.
- The 6-benzylaminopurine (BAP) treatment was optimized for sustained leaf viability and spore yield.
Main Results:
- Viable Puccinia coronata spores were produced across various treatments.
- The BAP treatment significantly enhanced leaf longevity and spore production.
- Detached oat leaves exhibited resistance reactions consistent with whole plants.
Conclusions:
- The developed detached-leaf system enables controlled propagation of single-race Puccinia coronata cultures.
- This system is effective for evaluating oat host resistance under standardized conditions.
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