Related Experiment Video
Updated: Jan 29, 2026

Pyrosequencing for Microbial Identification and Characterization
Published on: August 22, 2013
Identification and Partial Characterization of a New Luteovirus Associated with Rose Spring Dwarf Disease
Nidá Salem1, Deborah A Golino1, Bryce W Falk1
1Department of Plant Pathology, University of California, Davis 95616.
Abstract:
A number of viruses in the genera Ilarvirus and Nepovirus have been shown to be associated with specific diseases in rose, but many graft-transmissible rose diseases still have unknown etiologies. One of these diseases originally was detected by grafting from nonsymptomatic roses to Rosa multiflora indicator plants. Double-stranded RNAs (dsRNAs) were recovered and used as templates for cDNA synthesis and generating a cDNA library. Analysis of deduced amino acid sequences clearly positioned this virus as a member of the family Luteoviridae. The name rose spring dwarf associated virus (RSDaV) is tentatively proposed for the novel virus because the symptoms of this virus on R. multiflora are consistent with previous descriptions of rose spring dwarf disease (RSD). Phylogenetic analysis revealed a close relationship of RSDaV with members of the genus Luteovirus. Aphid transmission studies identified the rose-grass aphid (Metapolophium dirhodum) and yellow rose aphid (Rhodobium porosum) as vectors for this new virus. Host range data showed that RSDaV has a host range including both monocots and dicots. A specific reverse-transcription polymerase chain reaction assay was developed and revealed the presence of the RSDaV in several rose cultivars. RSDaV-inoculated rose plants developed RSD symptoms, confirming its role in the etiology of the disease.
Related Concept Videos
Frequency of Spring-Mass System
Consider a block on a spring on a frictionless surface. There...
Partial Fractions
Mixtures of Gases: Dalton's Law of Partial Pressures and Mole Fractions
Dalton's Law of Partial Pressure
Dosage Regimens: Partial Pharmacokinetic Parameters
Integration of Rational Functions Using Partial Fractions

