Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

626
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
626
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

4.5K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
4.5K
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

3.6K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
3.6K
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

1.3K
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
1.3K
Atomic Emission Spectroscopy: Interference01:30

Atomic Emission Spectroscopy: Interference

636
In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...
636
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

2.7K
Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Subtype-specific bone mineralization defects and early treatment amelioration in murine models of autosomal recessive osteopetrosis revealed by Raman spectroscopy.

Bone·2026
Same author

Hydroxy-Group Topology as a Molecular Trigger Between Antioxidant and Photosensitizing Properties in Dihydroxynaphthalenes.

ACS omega·2026
Same author

Nonequilibrium dynamics of high energy transitions in monolayer WSe<sub>2</sub>.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same author

Ultrafast Excited-State Dynamics of Dithienyltetrazine-Based Donor-Acceptor Copolymers.

The journal of physical chemistry. B·2026
Same author

Integration of 2D Materials in Radial van der Waals Heterostructure Metasurfaces.

ACS nano·2026
Same author

Coherent Electron-Phonon Coupling in Two-Dimensional Bi<sub>2</sub>Se<sub>3</sub> Nanoplatelets Studied with Ultrafast Spectroscopy.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

In This Issue.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Long-term cultural continuity across the Neanderthal-modern human sequence at Üçağızlı II Cave, northern Levant.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Dolphins use names to remember whom to avoid.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Retraction for Shaked and Frenkel, Curiouser and curiouser: Meningeal lymphoid structures in the aging brain.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Small but mighty: The outsized role of small water bodies in the global carbon cycle.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Functional traits produce conditional outcomes in different community contexts.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.6K

Single-molecule excitation-emission spectroscopy.

Erling Thyrhaug1, Stefan Krause2, Antonio Perri3

  • 1Dynamical Spectroscopy, Department of Chemistry, Technical University of Munich, 85748 Garching, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|February 17, 2019
PubMed
Summary
This summary is machine-generated.

Single-molecule spectroscopy now records excitation and emission spectra simultaneously. This breakthrough reveals uncorrelated environmental fluctuations in donor-acceptor dyads, advancing nanoscience research.

Keywords:
correlationsenergy transferfluorescencesingle moleculespectroscopy

More Related Videos

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
10:37

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

Published on: March 16, 2020

10.3K
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

9.4K

Related Experiment Videos

Last Updated: Jan 29, 2026

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.6K
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
10:37

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

Published on: March 16, 2020

10.3K
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

9.4K

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Single-molecule spectroscopy (SMS) offers unparalleled insight into individual molecular properties.
  • Collecting excitation spectra in SMS remains a significant technical hurdle.
  • Existing methods often rely on ensemble averaging, obscuring single-emitter details.

Purpose of the Study:

  • To develop a method for simultaneous collection of excitation and emission spectra from single molecules.
  • To overcome the challenge of recording excitation spectra in single-molecule spectroscopy.
  • To investigate the local nanoscopic environment of individual emitters, including donor-acceptor dyads.

Main Methods:

  • Implementation of a compact common-path interferometer as an extension to a standard SMS microscope.
  • Utilizing broadband excitation for simultaneous spectral data acquisition.
  • Simultaneous room-temperature collection of excitation and emission spectra from individual terrylene diimide molecules and donor-acceptor dyads in polystyrene.

Main Results:

  • Demonstration of simultaneous excitation and emission spectral collection from single molecules.
  • Analysis of spectral parameters using optical lineshape theory.
  • Detailed characterization of emitter-environment interactions at the single-molecule level.

Conclusions:

  • The developed technique successfully enables simultaneous excitation and emission spectral recording in SMS.
  • Optical lineshape analysis provides deep insights into molecular-environment interactions.
  • Environmental fluctuations between donor and acceptor moieties in dyads are found to be uncorrelated.