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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

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Expression pattern of microRNAs related with response to trastuzumab in breast cancer

Fan Yang1,2, Ziyi Fu1,3, Mengzhu Yang1,2

  • 1Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Journal of Cellular Physiology
|February 17, 2019
PubMed
Abstract

Insights

MicroRNAs (miRNAs) show promise as predictive biomarkers for trastuzumab response in HER2-positive breast cancer. This study identified specific miRNA expression patterns in resistant versus sensitive cell lines and patient serums, suggesting their potential in guiding treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Trastuzumab is a key therapy for HER2-positive breast cancer, but reliable biomarkers predicting response are lacking beyond HER2 status.
  • Identifying novel biomarkers is crucial for optimizing trastuzumab treatment strategies.

Purpose of the Study:

  • To investigate the association between microRNA (miRNA) expression profiles and trastuzumab resistance in HER2-positive breast cancer.
  • To identify potential miRNA biomarkers for predicting patient response to trastuzumab therapy.

Main Methods:

  • Microarray analysis identified differentially expressed miRNAs in trastuzumab-resistant versus sensitive cell lines.
  • Gene Ontology and KEGG pathway analyses elucidated the functions of identified miRNAs and their targets.
  • Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and receiver operating characteristic (ROC) curve analysis were used for validation and predictive performance evaluation in patient serum samples.

Main Results:

  • 151 differentially expressed miRNAs were identified between resistant and sensitive cell lines.
  • KEGG analysis implicated the PI3K-Akt signaling pathway.
  • Four miRNAs (miR-200b, miR-135b, miR-29a, and miR-224) showed significant correlation with trastuzumab resistance in patient serums.

Conclusions:

  • MicroRNAs demonstrate potential as reliable predictive biomarkers for trastuzumab response.
  • These findings may aid in personalizing trastuzumab treatment for HER2-positive breast cancer patients.
Keywords:
breast cancermiRNAtrastuzumab resistance

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