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MicroRNA-3191 promotes migration and invasion by downregulating TGFBR2 in colorectal cancer

Hongjuan He1,2, Xiaojuan Zhao1,2, Ziqing Zhu1,2

  • 1Key Laboratory of Resource Biology and Biotechnology, School of Life Sciences, Northwest University, Xi'an, China.

Journal of Biochemical and Molecular Toxicology
|February 17, 2019
PubMed

Insights

MicroRNA-3191 promotes colorectal cancer (CRC) cell migration and invasion by targeting the transforming growth factor beta receptor II (TGFBR2). This finding suggests miR-3191 as a potential therapeutic target for CRC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Transforming growth factor beta receptor II (TGFBR2) mutations occur in up to 30% of colorectal cancer (CRC) cases.
  • MicroRNA dysregulation is implicated in the pathogenesis of CRC.

Purpose of the Study:

  • To investigate the role of miR-3191 in colorectal cancer.
  • To determine if miR-3191 regulates the transforming growth factor beta (TGF-BETA) signaling pathway in CRC.
  • To explore miR-3191 as a potential therapeutic strategy for CRC.

Main Methods:

  • Bioinformatic analyses including Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathways, and Gene Set Enrichment Analysis.
  • In vitro experiments using CRC samples.
  • Luciferase reporter assays to confirm direct targeting.
  • Transwell migration and invasion assays to assess cell motility.

Main Results:

  • Bioinformatic analyses indicated miR-3191's involvement in the TGF-BETA signaling pathway in CRC.
  • miR-3191 was confirmed to target TGFBR2, modulating the TGF-BETA pathway.
  • miR-3191 promoted CRC cell migration and invasion by downregulating TGFBR2 expression.

Conclusions:

  • miR-3191 plays a significant role in CRC progression by regulating TGFBR2.
  • miR-3191 represents a potential novel therapeutic target for colorectal cancer treatment.
Keywords:
colorectal cancer (CRC)miR-3191migrationtransforming growth factor beta (TGF-BETA) signal pathwaytransforming growth factor beta receptor II (TGFBR2)

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