Short-term nitrogen dynamics are impacted by defoliation and drought in Fagus sylvatica L. branches

The predicted recurrence of adverse climatic events such as droughts, which disrupt nutrient accessibility for trees, could jeopardize the nitrogen (N) metabolism in forest trees. Internal tree N cycling capacities are crucial to ensuring tree survival but how the N metabolism of forest trees responds to intense, repeated environmental stress is not well known. For 2 years, we submitted 9-year-old beech (Fagus sylvatica L.) trees to either a moderate or a severe prolonged drought or a yearly removal of 75% of the foliage to induce internal N cycling changes. During the second year of stress, in spring and summer, we sprayed 15N-urea on the leaves (one branch per tree). Then, for 14 days, we traced the 15N dynamics through the leaves, into foliar proteins and into the branch compartments (leaves and stems segments), as well as its long-distance transfer from the labeled branches to the tree apical twigs. Defoliation caused a short- and mid-term N increase in the leaves, which remained the main sink for N. Whatever the treatment and the date, most of the leaf 15N stayed in the leaves and was invested in soluble proteins (60-68% of total leaf N). 15N stayed more in the proximal part of the branch in response to drought compared with other treatments. The long-distance transport of N was maintained even under harsh drought, highlighting efficient internal N recycling in beech trees. Under extreme constraints creating an N and water imbalance, compensation mechanisms operated at the branch level in beech trees and allowed them (i) to maintain leaf N metabolism and protein synthesis and (ii) to ensure the seasonal short- and long-distance transfer of recycled leaf N even under drastic water shortage conditions.