Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetics of Speciation02:16

Genetics of Speciation

21.0K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
21.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.9K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.6K
3.6K
What is Population Genetics?01:25

What is Population Genetics?

64.7K
A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.
64.7K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.0K
Overview
80.0K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.2K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A carboxylate switch point controls long-range energy transduction in respiratory Complex I.

Nature communications·2026
Same author

Enzyme-Free Phosphorylation with Kinetic Gating in a De Novo Coiled-Coil System.

Journal of the American Chemical Society·2026
Same author

Molecular Principles of Gating Proton Transport in the Antiporter Modules of Respiratory Complex I.

Journal of the American Chemical Society·2026
Same author

Low-barrier hydrogen bond powers long-range radical transfer in the metal-free ribonucleotide reductase.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Quinones operate as proton-collecting antennas in energy-transducing membranes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

The <i>Mycobacterium smegmatis bd</i>-II terminal oxidase employs a carboxylate shift mechanism.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Jan 29, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

1.9K

Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase.

Maximilian Fottner1, Andreas-David Brunner1, Verena Bittl2,3

  • 1Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry, Lab for Synthetic Biochemistry, Technical University of Munich, Institute for Advanced Study, TUM-IAS, Garching, Germany.

Nature Chemical Biology
|February 17, 2019
PubMed
Summary

Researchers developed a new method for inducible protein ubiquitylation in cells. This technique allows precise control over ubiquitylation events, aiding the study of protein modification functions.

More Related Videos

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

4.4K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

3.1K

Related Experiment Videos

Last Updated: Jan 29, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

1.9K
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

4.4K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

3.1K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Post-translational modification by ubiquitin and ubiquitin-like proteins (Ubls) regulates eukaryotic cellular processes.
  • Studying ubiquitylation is challenging due to difficulties in preparing homogeneous modified proteins and selectively triggering events in vivo.

Purpose of the Study:

  • To develop a general tool for inducible protein ubiquitylation.
  • To enable site-specific and ubiquitin-ligase-independent ubiquitylation in mammalian cells.

Main Methods:

  • Combination of genetic-code expansion, bioorthogonal Staudinger reduction, and sortase-mediated transpeptidation.
  • Generation of ubiquitin conjugates with native isopeptide bonds and resistance to deubiquitinases.
  • Site-specific attachment of Ubls to nonrefoldable, multidomain proteins.

Main Results:

  • Developed a tool for inducible protein ubiquitylation in mammalian cells.
  • Generated ubiquitin conjugates resistant to deubiquitinases while retaining native function.
  • Enabled temporal control over ubiquitylation events independent of endogenous ubiquitin ligases.

Conclusions:

  • The developed method provides a powerful tool for dissecting the biological functions of ubiquitylation with temporal control.
  • Facilitates the study of ubiquitylation on complex, nonrefoldable proteins.
  • Overcomes limitations in current ubiquitylation research methodologies.