Related Experiment Video
Updated: Jan 29, 2026
08:26
Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
Published on: October 19, 2015
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Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Endosome and Lysosome Isolation from Eukaryotic Cell.
Deepak B Thimiri Govinda Raj1, Niamat Ali Khan2, Srisaran Venkatachalam3
1Envirotransgene® Bio-solutions Global Chennai India and Institute of Cancer Research, Oslo University Hospital, Oslo, Norway. deepak.balaji@bitsaa.org.
Methods in Molecular Biology (Clifton, N.J.)
|February 17, 2019
Summary
We developed a new method using superparamagnetic nanoparticles (SPMNPs) to isolate pure lysosomes from HeLa cells. This technique enables detailed studies of lysosomes for better understanding of cellular functions.
Area of Science:
- Cell Biology
- Nanotechnology
- Biochemistry
Background:
- Lysosomes are crucial cellular organelles involved in degradation and recycling.
- Efficient isolation of lysosomes is essential for studying their function in health and disease.
- Current isolation methods can be complex and yield low purity.
Purpose of the Study:
- To develop a novel protocol for high-purity, high-yield lysosome isolation.
- To utilize superparamagnetic nanoparticles (SPMNPs) for efficient subcellular fractionation.
- To enable advanced proteomic and lipidomic analyses of isolated lysosomes.
Main Methods:
- Synthesis of iron oxide (Fe3O4) core SPMNPs.
- Surface functionalization of SPMNPs with dimercaptosuccinic acid (DMSA) to create DMSA-SPMNPs.
- Application of DMSA-SPMNPs for the isolation of endosomes and lysosomes from HeLa cells.
Main Results:
- Successful synthesis of DMSA-SPMNPs.
- Demonstration of DMSA-SPMNPs for effective isolation of endosomes and lysosomes.
- Achieved high-purity and high-yield isolation of lysosomes.
Conclusions:
- The SPMNP-based protocol provides an efficient method for lysosome isolation.
- This technique facilitates in-depth proteomic and lipidomic studies of lysosomes.
- Improved understanding of lysosome function through advanced molecular analysis.
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