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Updated: Jan 29, 2026

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Published on: February 16, 2017
Fluorescent Ag clusters conjugated with anterior gradient-2 antigen aptamer for specific detection of cancer cells
Jinze Lan1, Xiaoxia Wu2, Liang Luo2
1Laboratory of Environmental Sciences and Technology, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, Xinjiang 830011, China; University of Chinese Academy of Sciences, No. 19 Yuquan Road, Beijing 100049, China.
Abstract:
Anterior gradient protein 2 homolog (AGR) is a potential tumor biomarker and plays an important role in tissue development and regeneration. The intracellular detection of AGR is rarely reported. By conjugating the AGR aptamer with a cytosine base sequence as Ag cluster template, a highly fluorescent probe (MA@AgNCs) was synthesized for targeting intracellular AGR. The MA@AgNCs display a maximum fluorescence peak at 565 nm, and possess an excellent quantum yield (QY = 87.43%), small size, great biocompatibility, low toxicity, and good stability. Moreover, the as synthesized MA@AgNCs show high specificity on recognizing breast cancer cells.
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