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Macromolecular inversion-driven polymer insertion into model lipid bilayer membranes.

Sivaramakrishnan Ramadurai1, Ananiy Kohut2, Nirod Kumar Sarangi1

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|February 18, 2019
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Summary
This summary is machine-generated.

Amphiphilic invertible polymers (AIPs) can self-assemble into micelles for drug delivery. This study shows AIPs alter lipid membrane permeability and fluidity, with more hydrophobic polymers having a greater impact, aiding future membrane delivery applications.

Keywords:
1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)Amphiphilic invertible polymer micellesDiffusion coefficientElectrochemical impedance spectroscopy (EIS)Fluorescence lifetime correlation spectroscopy (FLCS)ImpedanceLipid probesMicrocavity supported lipid bilayer (MSLB)

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Area of Science:

  • Polymer Science
  • Materials Science
  • Biophysics

Background:

  • Amphiphilic invertible polymers (AIPs) self-assemble into micelles, useful for encapsulating and releasing cargo.
  • These micelles show potential for drug delivery, particularly for poorly water-soluble compounds.
  • Limited research exists on AIP interactions with lipid membranes.

Purpose of the Study:

  • To investigate the interaction of three AIPs with varying hydrophobicity/hydrophilicity balance with a lipid bilayer.
  • To understand how AIP micellar assemblies affect lipid membrane permeability and fluidity.
  • To explore the potential of AIPs for membrane molecular delivery.

Main Methods:

  • Utilized electrochemical impedance spectroscopy (EIS) to measure bilayer permeability.
  • Employed fluorescence correlation spectroscopy (FCS) to assess bilayer fluidity.
  • Investigated AIPs at concentrations above and below their critical micelle concentrations (cmcs).

Main Results:

  • All tested AIPs increased membrane permeability and decreased fluidity at concentrations above their cmcs.
  • The degree of impact on the lipid bilayer was dependent on the AIP's hydrophobicity.
  • The most hydrophobic AIP, PEG600-PTHF650, significantly altered bilayer properties and demonstrated micelle inversion and cargo release at the membrane.

Conclusions:

  • AIPs influence the physical properties of lipid bilayers, with hydrophobicity being a key factor.
  • AIP micelle inversion and cargo release at the membrane are feasible.
  • Findings provide insights for developing AIPs for advanced membrane molecular delivery systems.