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Related Experiment Video

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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

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Fast, volumetric live-cell imaging using high-resolution light-field microscopy.

Haoyu Li^1,2, Changliang Guo³, Deborah Kim-Holzapfel⁴

¹Department of Biomedical Engineering, Stony Brook University (SUNY), Stony Brook, New York, USA.

Biomedical Optics Express

|February 19, 2019

Summary

High-resolution light-field microscopy (HR-LFM) achieves subcellular resolution for live-cell imaging. This breakthrough enables millisecond volumetric imaging, advancing biological systems research across multiple scales.

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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

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High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

Published on: April 12, 2012

Area of Science:

Cell biology
Microscopy
Biophysics

Background:

Visualizing anatomical and functional traits across spatial scales requires high spatio-temporal resolution.
Light-field microscopy (LFM) offers scanning-free, scalable, high-speed volumetric imaging, particularly for brain function.
Current LFM faces challenges in achieving subcellular, near-diffraction-limited 3D resolution for single-cell studies.

Purpose of the Study:

To develop and demonstrate a high-resolution light-field microscopy (HR-LFM) technique for live-cell imaging.
To achieve subcellular spatial resolution (300-700 nm) in 3D with high temporal resolution (milliseconds).
To enable advanced observation of cellular dynamics, structures, and particle tracking.

Main Methods:

Implementation of a novel high-resolution light-field microscopy (HR-LFM) system.
Live-cell imaging experiments utilizing the HR-LFM technique.
Demonstration through imaging of cellular dynamics, structures, and single particle tracking.

Main Results:

Achieved 3D spatial resolution of 300-700 nm in live cells.
Enabled volumetric imaging with millisecond acquisition times.
Successfully imaged diverse cellular dynamics, structures, and tracked single particles.

Conclusions:

HR-LFM significantly enhances the capabilities of light-field microscopy for live-cell studies.
The technique provides unprecedented 3D subcellular resolution and speed.
HR-LFM is poised to become a valuable tool for understanding biological systems at multiple spatio-temporal levels.