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Updated: Jan 29, 2026
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
18.9K
Integrated multimodal photoacoustic microscopy with OCT- guided dynamic focusing
Arash Dadkhah1, Jun Zhou2, Nusrat Yeasmin1
1Department of Biomedical Engineering, Florida International University, Miami, FL 33174, USA.
Biomedical Optics Express
|February 19, 2019
Summary
We developed a multimodal microscopic optical imaging system integrating photoacoustic microscopy, optical coherence tomography, optical Doppler tomography, and confocal fluorescence microscopy. This system offers comprehensive structural, functional, and molecular information for biological tissues.
Area of Science:
- Biomedical Optics
- Microscopy
- Medical Imaging
Background:
- Simultaneous multimodal imaging is highly desirable for comprehensive biological tissue analysis.
- Integrating diverse imaging modalities presents significant optical and hardware challenges.
Purpose of the Study:
- To develop a single multimodal microscopic optical imaging platform.
- To provide simultaneous structural, functional, and molecular information from living tissues.
Main Methods:
- Integrated photoacoustic microscopy (PAM), optical coherence tomography (OCT), optical Doppler tomography (ODT), and confocal fluorescence microscopy.
- Developed a novel OCT-guided surface contour scanning for dynamic focusing adjustment.
Main Results:
- Demonstrated the system's capability for comprehensive microscopic imaging of biological tissues.
- Successfully conducted phantom, in vivo, and ex vivo tests.
Conclusions:
- The developed multimodal imaging system offers a powerful tool for preclinical research.
- OCT-guided dynamic focusing enhances simultaneous multimodal imaging capabilities for future clinical applications.
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