Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methods for concentration of urinary immunoreactive insulin.

B H Du1, J Eng, R S Yalow

  • 1Solomon A. Berson Research Laboratory, Veterans Administration Medical Center, Bronx, New York 10468.

Journal of Korean Medical Science
|September 1, 1986
PubMed
Summary

Urine insulin can be concentrated using C18 Sep-Pak cartridges for radioimmunoassay measurement. This method aids in determining renal insulin clearance and urinary insulin output in normal subjects.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of the pathogenic spectrum and epidemiological characteristics of acute respiratory infection in Henan Province from 2024 to 2025].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2026
Same author

[Construction and application of the evaluation system for the risk of measles transmission in Henan Province].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025
Same author

[Epidemiological and molecular traceability analysis of the first cluster outbreak of D8 genotype measles in Henan Province].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025
Same author

The photochemistry of Rydberg-excited cyclobutanone: Photoinduced processes and ground state dynamics.

The Journal of chemical physics·2024
Same author

COVID-19 in patients with lung cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2020
Same author

Understanding the potential for efficient triplet harvesting with hot excitons.

Faraday discussions·2019

Area of Science:

  • Endocrinology
  • Nephrology
  • Biochemistry

Background:

  • Insulin measurement in biological fluids is crucial for understanding glucose metabolism and endocrine function.
  • Assessing urinary insulin levels can provide insights into insulin secretion and renal handling.
  • Existing methods for urinary insulin analysis may require significant sample volumes or lack efficiency.

Purpose of the Study:

  • To develop and validate a method for concentrating insulin from urine for subsequent analysis.
  • To determine normal ranges for renal insulin clearance and urinary insulin output in non-diabetic subjects.
  • To explore the utility of urinary insulin concentration for identifying abnormal insulin variants.

Main Methods:

  • Concentration of insulin from urine samples (10-50 ml) using octadecylsilyl (ODS) silica columns (C18Sep-Pak cartridge).

Related Experiment Videos

  • Measurement of concentrated insulin by radioimmunoassay.
  • Fractionation of urinary insulin using Sephadex G50 gel filtration.
  • Determination of renal insulin clearance in normal subjects under fasted and fed conditions.

    • Main Results:

    • Urine insulin recovery exceeded 75% using ODS silica columns.
    • Fractionation indicated that the majority of immunoreactivity corresponded to 6000 dalton insulin.
    • Renal insulin clearance in normal subjects averaged 0.44 +/- 0.10 ml/min, irrespective of feeding state.
    • Urinary insulin output increased post-feeding and decreased during prolonged fasting, averaging 25 +/- 10 mU/24 hr in non-diabetics.
    • Renal insulin clearance and timed urinary output were sufficient for estimating mean plasma insulin in subjects without renal disease.

    Conclusions:

    • Octadecylsilyl silica columns provide an efficient method for concentrating urinary insulin for radioimmunoassay.
    • The developed method allows for the assessment of renal insulin clearance and urinary insulin output, establishing normal reference ranges.
    • This technique holds potential for screening and purifying abnormal insulin variants through HPLC analysis.