Denitrification as a major regional nitrogen sink in subtropical forest catchments: Evidence from multi-site dual...

Increasing nitrogen (N) deposition in subtropical forests in south China causes N saturation, associated with significant nitrate (NO₃^- ) leaching. Strong N attenuation may occur in groundwater discharge zones hydrologically connected to well-drained hillslopes, as has been shown for the subtropical headwater catchment "TieShanPing", where dual NO₃^- isotopes indicated that groundwater discharge zones act as an important N sink and hotspot for denitrification. Here, we present a regional study reporting inorganic N fluxes over two years together with dual NO₃^- isotope signatures obtained in two summer campaigns from seven forested catchments in China, representing a gradient in climate and atmospheric N input. In all catchments, fluxes of dissolved inorganic N indicated efficient conversion of NH₄⁺ to NO₃^- on well-drained hillslopes, and subsequent interflow of NO₃^- over the argic B-horizons to groundwater discharge zones. Depletion of ¹⁵ N- and ¹⁸ O-NO₃^- on hillslopes suggested nitrification as the main source of NO₃^- . In all catchments, except one of the northern sites, which had low N deposition rates, NO₃^- attenuation by denitrification occurred in groundwater discharge zones, as indicated by simultaneous ¹⁵ N and ¹⁸ O enrichment in residual NO₃^- . By contrast to the southern sites, the northern catchments lack continuous and well-developed groundwater discharge zones, explaining less efficient N removal. Using a model based on ¹⁵ NO₃^- signatures, we estimated denitrification fluxes from 2.4 to 21.7 kg N ha^-1 year^-1 for the southern sites, accounting for more than half of the observed N removal. Across the southern catchments, estimated denitrification scaled proportionally with N deposition. Together, this indicates that N removal by denitrification is an important component of the N budget of southern Chinese forests and that natural NO₃^- attenuation may increase with increasing N input, thus partly counteracting further aggravation of N contamination of surface waters in the region.