Related Experiment Video
Updated: Jan 29, 2026

Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes
Published on: January 10, 2022
A novel fluorimetric method for glyphosate and AMPA determination with NBD-Cl and MCR-ALS
Ana L Pérez1, Giuliana Tibaldo2, Germán H Sánchez3
1Universidad Nacional del Litoral, Facultad de Bioquímica y Ciencias Biológicas, Departamento de Física, Ciudad Universitaria, S3000ZAA Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, C1425FQB Ciudad Autónoma de Buenos Aires, Argentina.
Abstract:
We report the development of a new analytical method for the quantification of N-(phosphonomethyl)glycine (glyphosate) and (aminomethyl)phosphonic acid (AMPA) by combining spectrofluorimetry and multivariate calibration. In this study, fluorescence spectroscopy was used to quantify glyphosate and AMPA, which were previously derivatized with the fluorogenic reagent: 4-chloro-7-nitrobenzofurazan (NBD-Cl). Fluorescence excitation-emission matrices (EEM) were recorded by exciting between 400 and 500 nm, and measuring the emission between 500 and 610 nm. The second-order data obtained were processed using the Multivariate Curve Resolution with Alternating Least Square (MCR-ALS) methodology. The developed method was used to predict different concentrations of glyphosate and AMPA in validation samples. In addition, the presence of the herbicide was evaluated in real samples: a commercial formulation and a water sample from a cultivated area. For this purpose, the standard addition method was used to study the matrix effect in each case. The ranges of working concentrations obtained for this new method are in agreement with the amounts found in surface water samples near a direct sowing soybean growing region in Argentina.
Related Concept Videos
Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
Dose Size and Dosing Frequency: Determination Methods
Dosage Interval and Administration Route: Determination Methods
Determination of Renal Drug Clearance: Graphical and Midpoint Methods
The graphical method involves plotting the rate of drug excretion in urine against the plasma drug concentration. By analyzing the graph, the clearance can be calculated and obtained. Drugs rapidly excreted by the kidneys exhibit a...
Determining Order of Reaction
Determination

