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Lab-on-a-brane for spheroid formation.

M Bianco1, A Zizzari1,2, P Priore1

  • 1CNR-NANOTEC, Institute of Nanotechnology c/o Campus Ecotekne, Lecce, Italy.

Biofabrication
|February 19, 2019
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Summary
This summary is machine-generated.

Lab-On-a-Brane (LOB) technology promotes 3D cell growth in cancer cell biochips. LOBs offer advantages over traditional methods by enhancing cell aggregation and enabling new cellular biology studies.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Materials Science

Background:

  • Lab-On-a-Chip (LOC) technology offers microfluidic advantages for cell culture.
  • Integrating flexible, gas-permeable membranes (TMs) into LOCs creates Lab-On-a-Brane (LOB) systems.
  • LOBs aim to enhance cell culture capabilities beyond traditional multiwells (MWs).

Purpose of the Study:

  • To investigate the potential of LOBs as cell biochips for promoting 3D cell growth.
  • To compare 3D cell aggregation in LOBs with conventional MWs and standard LOCs.
  • To evaluate the influence of substrate wettability and membrane properties on cell behavior.

Main Methods:

  • Human cancer cells (MCF-7) were cultured in standard MWs, polydimethylsiloxane (PDMS) MWs, LOCs, and LOBs.
  • Surface treatments (oxygen plasma, coating deposition) were used to create hydrophilic, hydrophobic, and oleophobic chips.
  • 3D cell aggregation was monitored and compared across different chip types and wettabilities.

Main Results:

  • 3D cell aggregation was significantly favored in LOBs, irrespective of substrate wettability.
  • The enhanced aggregation in LOBs is attributed to the TM flexibility and high gas permeability (O2/CO2).
  • LOBs combine the operational benefits of LOCs with the mechanical and morphological advantages of PDMS TMs.

Conclusions:

  • LOBs show promise as advanced cell biochips that promote 3D cell growth.
  • The unique properties of LOBs facilitate studies on cell mechanics, metabolism, and responses to stimuli.
  • LOB technology integrates microfluidic efficiency with biomembrane characteristics for novel cellular biology research.