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Sensitive and immunogen-specific serological detection of Rodentibacter pneumotropicus infections in mice

Felix Fingas1,2, Daniela Volke1,3, Rayk Hassert1,3

  • 1Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Leipzig, Germany.

BMC Microbiology
|February 20, 2019
PubMed
Abstract

Insights

A new protein antigen, CARLO-1, enables sensitive and specific detection of Rodentibacter pneumotropicus infections in mice. This advancement improves upon current assays, which often yield false negatives, ensuring better animal health monitoring.

Area of Science:

  • Veterinary Microbiology
  • Immunology
  • Animal Health

Background:

  • Rodentibacter pneumotropicus is a common respiratory and urogenital pathogen in laboratory mice.
  • Current serological assays for R. pneumotropicus exhibit limitations in sensitivity, leading to potential misdiagnosis.
  • Accurate detection is crucial for maintaining the health and integrity of research animal populations.

Purpose of the Study:

  • To develop a highly sensitive and specific diagnostic system for Rodentibacter infections in mice.
  • To identify key antigens for differentiating R. pneumotropicus from closely related species like R. heylii.
  • To improve upon existing serological testing methods for laboratory animal pathogens.

Main Methods:

  • Systematic analysis of protein and lipopolysaccharide antigens using immunoblot and ELISA.
  • Identification of the main immunogen (CARLO-1) via 2D gel electrophoresis, immunoblot, and mass spectrometry.
  • Recombinant expression of CARLO-1 for developing an indirect ELISA.

Main Results:

  • A sensitive test system was established to differentiate R. pneumotropicus and R. heylii.
  • The characteristic antigen for Rodentibacter of laboratory origin 1 (CARLO-1) was identified as the main immunogen.
  • An indirect ELISA using recombinant CARLO-1 demonstrated high sensitivity, specificity, and selectivity, outperforming a commercial ELISA.

Conclusions:

  • The newly identified CARLO-1 protein is suitable for sensitive and specific serological detection of Rodentibacter infections.
  • ELISA systems utilizing CARLO-1 or whole-cell preparations offer superior sensitivity compared to commercial assays.
  • Improved diagnostic tools are essential for accurate identification and management of R. pneumotropicus in laboratory mice.
Keywords:
AntigenCARLO-1ELISAFELASAHealth monitoringPasteurella pneumotropicaRodentibacter heyliiRodentibacter pneumotropicusTwo-dimensional gel electrophoresis

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