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High-fat and high-glucose microenvironment decreases Runx2 and TAZ expression and inhibits bone regeneration in the

Xuan Wu1,2, Yunpeng Zhang1,2, Yixiao Xing1,2

  • 1Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, 250012, Shandong, People's Republic of China.

Journal of Orthopaedic Surgery and Research
|February 20, 2019
PubMed
Abstract

Insights

High-fat and high-glucose conditions impair bone regeneration. This may be linked to reduced expression of key proteins, runt-related transcription factor 2 (Runx2) and TAZ, crucial for bone healing.

Area of Science:

  • Biomedical research
  • Bone biology
  • Metabolic disease research

Background:

  • Type 2 diabetes mellitus (T2DM) and hyperlipidemia negatively impact bone regeneration.
  • Understanding the effects of metabolic derangements on bone healing is critical.

Purpose of the Study:

  • To investigate the impact of a high-fat and high-glucose environment on mandibular bone regeneration.
  • To assess the expression of runt-related transcription factor 2 (Runx2) and transcriptional co-activator with PDZ-binding domain (TAZ) during this process.

Main Methods:

  • A high-fat and high-glucose mouse model was used with a mandibular bone defect.
  • Bone regeneration was evaluated using histological staining (H&E, Masson, TRAP) and immunohistochemistry.
  • Runx2 and TAZ expression levels were quantified via RT-PCR and Western blot analysis.

Main Results:

  • The high-fat and high-glucose group exhibited the most significant inhibition of bone regeneration.
  • Expression of Runx2 was reduced in high-fat, high-glucose, and combined groups compared to controls.
  • TAZ expression showed a decreasing trend specifically in the high-fat and high-glucose group.

Conclusions:

  • A high-fat and high-glucose microenvironment significantly inhibits bone regeneration.
  • This inhibition may be associated with the downregulation of Runx2 and TAZ expression.
  • These findings highlight potential molecular mechanisms underlying impaired bone healing in metabolic disorders.
Keywords:
Bone regenerationHyperlipidemiaRunx2 (runt-related transcription factor 2)T2DM (type 2 diabetes mellitus)TAZ (transcriptional co-activator with PDZ-binding domain)

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