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An effective mutagenic method in Azotobacter vinelandii
F Luque1, E Santero, J R Medina
1Departamento de Genética, Facultad de Biología, Universidad de Sevilla.
Microbiologia (Madrid, Spain)
|February 1, 1987
Abstract:
The acridine-like compound ICR-191 is an effective mutagenic agent in Azotobacter vinelandii. Selectable mutants, such as those resistant to chlorate, can be isolated without post-mutagenic segregation. Non selectable mutants, such as those unable to metabolize different sugars, can be easily isolated after twelve generations of post-mutagenic segregation.