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Updated: Jan 29, 2026

Assays for Validating Histone Acetyltransferase Inhibitors
Published on: August 6, 2020
Gcn5 histone acetyltransferase is present in the mitoplasts
Arianna Montanari1,2, Manuela Leo3, Veronica De Luca3
1Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy ari.montanari@uniroma1.it.
Abstract:
In Saccharomyces cerevisiae the Lysine-acetyltransferase Gcn5 (KAT2) is part of the SAGA complex and is responsible for histone acetylation widely or at specific lysines. In this paper we report that G CN5 deletion differently affects the growth of two strains. The defective mitochondrial phenotype is related to a marked decrease in mtDNA content, which also involves the deletion of specific regions of the molecule. We also show that in wild-type mitochondria the Gcn5 protein is present in the mitoplasts, suggesting a new mitochondrial function independent from the SAGA complex and possibly a new function for this protein connecting epigenetics and metabolism.
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