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LncSNHG14 promotes the development and progression of bladder cancer by targeting miRNA-150-5p
1Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou, China. shanyuxiv@163.com.
Objective:
To elucidate whether lncSNHG14 could influence the proliferative potential and cell cycle progression of bladder cancer cells via binding to microRNA-150-5p (miRNA-150-5p). We aim to investigate the potential mechanism of miRNA-150-5p in the occurrence and progression of bladder cancer (BCa).
Patients And Methods:
Expression levels of SNHG14 and miRNA-150-5p in BCa tissues and normal bladder tissues were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Their expressions in BCa cell lines were detected as well. Regulatory effects of NHG14 and miRNA-150-5p on proliferative potential and cell cycle progression were evaluated by cell counting kit-8 (CCK-8) and flow cytometry, respectively. Through the dual-luciferase reporter gene assay, binding conditions between SNHG14 and miRNA-150-5p, as well as between miRNA-150-5p and synaptic vesicle-associated membrane protein 2 (VAMP2), were verified. Finally, rescue experiments were performed to clarify whether SNHG14 regulated behaviors of BCa cells by absorbing miRNA-150-5p to degrade VAMP2.
Results:
SNHG14 was highly expressed in BCa tissues and cell lines. The overexpression of SNHG14 accelerated the proliferative potential and cell cycle progression of BCa cells. SNHG14 was confirmed to bind to miRNA-150-5p. MiRNA-150-5p remained a low expression in BCa tissues. Moreover, miRNA-150-5p overexpression suppressed proliferative potential and cell cycle progression of BCa cells, which could reverse the promotive role of SNHG14 on behaviors of BCa cells. Furthermore, VAMP2 was the target gene of miRNA-150-5p. VAMP2 overexpression reversed the biological function of miRNA-150-5p in inhibiting proliferative potential and cell cycle progression of T24 and UC9 cells.
Conclusions:
LncSNHG14 overexpression accelerates proliferative potential and cell cycle progression of BCa cells through absorbing miRNA-150-5p to degrade VAMP2 expression.
Insights
Long non-coding RNA SNHG14 promotes bladder cancer (BCa) cell proliferation and cell cycle progression. It achieves this by binding to microRNA-150-5p (miRNA-150-5p), leading to the degradation of VAMP2 expression.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Bladder cancer (BCa) is a significant health concern with complex molecular underpinnings.
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development and progression.
- Understanding the regulatory mechanisms, such as lncRNA-microRNA interactions, is crucial for targeted therapies.
Purpose of the Study:
- To investigate the role of lncSNHG14 in bladder cancer (BCa) cell proliferation and cell cycle progression.
- To determine if lncSNHG14 influences BCa cells by interacting with microRNA-150-5p (miRNA-150-5p).
- To elucidate the downstream molecular targets and mechanisms involved in this interaction.
Main Methods:
- Quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR) to assess SNHG14 and miRNA-150-5p expression in BCa tissues and cell lines.
- Cell Counting Kit-8 (CCK-8) and flow cytometry to evaluate the effects of SNHG14 and miRNA-150-5p on cell proliferation and cell cycle.
- Dual-luciferase reporter gene assay to confirm binding interactions between SNHG14, miRNA-150-5p, and VAMP2.
- Rescue experiments to validate the regulatory pathway.
Main Results:
- lncSNHG14 was significantly upregulated in BCa tissues and cell lines.
- Overexpression of SNHG14 enhanced BCa cell proliferation and accelerated cell cycle progression.
- SNHG14 directly binds to miRNA-150-5p, which was found to be downregulated in BCa.
- Overexpression of miRNA-150-5p inhibited BCa cell proliferation and cell cycle, counteracting SNHG14's effects.
- VAMP2 was identified as a direct target gene of miRNA-150-5p, and its overexpression reversed miRNA-150-5p's inhibitory effects.
Conclusions:
- lncSNHG14 promotes bladder cancer cell proliferation and cell cycle progression.
- This oncogenic effect is mediated through the sponging of miRNA-150-5p, leading to the upregulation of its target gene, VAMP2.
- Targeting the lncSNHG14/miRNA-150-5p/VAMP2 axis may offer a therapeutic strategy for bladder cancer.
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