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Long noncoding RNA AK027294 acts as an oncogene in non-small cell lung cancer by up-regulating STAT3

B Chen1, C-H Ling

  • 1Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China. linchunhua88@hotmail.com.

European Review for Medical and Pharmacological Sciences
|February 20, 2019
PubMed
Abstract

Insights

Long noncoding RNA AK027294 promotes non-small cell lung cancer (NSCLC) growth by up-regulating signal transducers and activators of transcription 3 (STAT3). AK027294 may serve as a therapeutic target for NSCLC treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in various diseases, including cancers.
  • Understanding the role of specific lncRNAs in tumorigenesis is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the role of lncRNA AK027294 in the development of non-small cell lung cancer (NSCLC).
  • To elucidate the underlying molecular mechanism of AK027294 in NSCLC progression.

Main Methods:

  • Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) to assess AK027294 expression.
  • Cell proliferation assays (CCK-8, colony formation, EdU) to evaluate NSCLC cell growth.
  • Western blot and RT-qPCR to analyze the expression of STAT3.

Main Results:

  • AK027294 expression was significantly elevated in NSCLC tissues compared to adjacent tissues.
  • Silencing AK027294 inhibited NSCLC cell growth and proliferation.
  • Knockdown of AK027294 led to decreased expression of signal transducers and activators of transcription 3 (STAT3).
  • AK027294 expression positively correlated with STAT3 expression in NSCLC.

Conclusions:

  • AK027294 promotes NSCLC cell growth by up-regulating STAT3.
  • AK027294 represents a potential therapeutic target for NSCLC.

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