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Published on: November 27, 2014
Characterization of Galectin-2 from Nile tilapia (Oreochromis niloticus) involved in the immune response to bacterial
Jinzhong Niu1, Yu Huang2, Jimin Niu1
1College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
Abstract:
galectin-2 plays important roles in innate and adaptive immunity. In this study, galectin-2 (OnGal-2) was identified from Nile tilapia (Oreochromis niloticus). Its tissue distribution and expression patterns following bacterial infection were also investigated. OnGal-2 is widely distributed in various tissues of healthy tilapia. After Streptococcus agalactiae challenge, OnGal-2 expressions were significantly up-regulated in all tested tissues. Meanwhile, the recombinant OnGal-2 (rOnGal-2) protein showed strong agglutinating activities against both Gram-negative bacteria and Gram-positive bacteria. Moreover, rOnGal-2 could promote phagocytosis of macrophages. Taken together, the present study indicated that OnGal-2 might play roles in the immune responses of Nile tilapia against bacterial pathogens.
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