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Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
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Engineering human ventricular heart tissue based on macroporous iron oxide scaffolds.

Hui Yang1, Lai Wei2, Chen Liu2

  • 1Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Shanghai Key Laboratory of Clinical Geriatric Medicine, Fudan University, Shanghai 200032, China; Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

Acta Biomaterialia
|February 20, 2019
PubMed
Summary

Engineered heart tissue (EhVHT) using stem cells and iron oxide scaffolds successfully repaired heart function in rats with myocardial infarction (MI). This ventricular-specific tissue shows promise for treating heart damage.

Keywords:
Engineered heart tissuesIron oxide scaffoldsMyocardial infarctionVentricular myocytes

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cardiovascular Research

Background:

  • Myocardial infarction (MI) is a leading cause of death globally, necessitating advanced therapeutic strategies.
  • Engineered heart tissues (EHTs) offer a promising solution for myocardial repair, with ventricular-specific tissues being particularly desirable.
  • Current treatments for MI often fall short, highlighting the need for innovative approaches to restore cardiac function.

Purpose of the Study:

  • To engineer a functional, ventricular-specific human heart tissue (EhVHT) for treating myocardial infarction.
  • To evaluate the in vitro and in vivo efficacy of the developed EhVHT in repairing cardiac damage.
  • To assess the potential of EhVHT for therapeutic applications and drug screening for ventricular myocardium.

Main Methods:

  • Constructed EhVHT by combining a 3D iron oxide scaffold (IOS) with human pluripotent stem cell (hPSC)-derived ventricular cardiomyocytes and mesenchymal stem cells.
  • Assessed EhVHT's cardiac-specific gene expression, ion exchange, calcium handling, and electrophysiological activity in vitro.
  • Evaluated EhVHT's efficacy in vivo by patching it onto the infarcted area of rat hearts with acute MI.

Main Results:

  • The developed EhVHT demonstrated enhanced expression of cardiac-specific genes and improved ion exchange.
  • EhVHT exhibited superior Ca2+ handling behaviors and normal electrophysiological activity in vitro.
  • In vivo studies showed that EhVHT effectively promoted heart tissue repair and restored cardiac function in a rat MI model.

Conclusions:

  • It is feasible to generate functional human ventricular heart tissue using hPSC-derived ventricular myocytes and IOS scaffolds.
  • The engineered human ventricular-specific heart tissue (EhVHT) shows significant potential for treating ventricular myocardial damage.
  • EhVHT holds promise for both therapeutic applications in MI treatment and as a platform for drug screening targeting ventricular myocardium.