First Record of the Perfect Stage Calonectria pauciramosa on Mastic Tree in Italy

Leaf spots, stem lesions, crown rot, and root rot, caused by heterothallic fungus Cylindrocladium pauciramosum C.L. Schoch & Crous,, on young plants in a nursery represent an important problem in many representative ornamental plant-growing areas of Italy since the disease was first reported in 1993 (2). Symptoms observed during surveys in Italy from 1993 to 2005 were attributed solely to the asexual stage, and the teleomorph (Calonectria pauciramosa C.L. Schoch & Crous) was never found. In October 2005, orange-to-red brown fungal fruiting bodies pertaining to the genus Calonectria were observed in belowground regions and at the soil line on collar and stem tissues of seedlings of the mastic tree (Pistacia lentiscus L., Anacardiaceae) affected by damping off in two nurseries of eastern Sicily. The sexual structures were solitary or in groups, orange-to-red brown, subglobose to ovoid. On the basis of 50 microscopic observations of leaf tissues, they ranged from 180 to 280 μm in diameter and from 270 to 400 μm high. In the presence of adequate moisture, ascospores were discharged from mature perithecia in visible, viscous, and white droplets. Asci containing eight ascospores were clavate and tapering to a long thin stalk. Fusoid ascospores were hyaline and guttulate with rounded ends, slightly curved, 1-septate, not or slightly constricted at the septum, and measured 33 to 39 μm long and 6 to 8 μm wide. However, measurements show that perithecia, asci, and ascospores also fall within the range described by Schoch et al. (3). Single ascospore cultures did not produce perithecia. On the basis of these descriptions and the ability of single-ascospore cultures to mate with two Italian (DISTEF-G87 and DISTEF-G128) and two South African (STE-U 971 and STE-U 1670) selected tester strains of Cylindrocladium pauciramosum (2), the perithecia were identified as C. pauciramosa. To our knowledge, this is the first natural occurrence of the perfect stage of Cylindrocladium pauciramosum in Italy. The presence of the teleomorph is very important because it represents a second means of spread after conidia. Furthermore, the occurrence of the teleomorph also could lead to a 1:1 mating type ratio. In this case, lower percentages of hermaphrodites should be expected (1,4). References: (1) G. Polizzi et al. J. Plant Pathol. 80:262, 1998. (2) G. Polizzi and P. W. Crous. Eur. J. Plant Pathol. 105:413, 1999. (3) C. L. Schoch et al. Mycologia 91:286, 1999. (4) C. L. Schoch et al. Plant Dis. 85:941, 2001.