Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Composition of Blood Plasma01:24

Composition of Blood Plasma

8.4K
Blood plasma is a fluid that contains approximately 92% water and 8% solutes. The solutes include various types of proteins, which constitute about 7% of the total solutes in the plasma. The high-molecular-weight proteins—albumins, globulins, and fibrinogen—are essential to plasma function. Albumins, making up about 60% of the plasma proteins, maintain the osmotic balance within blood vessels by preventing excessive water leakage. Additionally, albumins serve as carrier proteins,...
8.4K
Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

2.3K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
2.3K
Activation Energy01:26

Activation Energy

86.6K
Activation energy is the minimum amount of energy necessary for a chemical reaction to move forward. The higher the activation energy, the slower the rate of the reaction. However, adding heat to the reaction will increase the rate, since it causes molecules to move faster and increase the likelihood that molecules will collide. The collision and breaking of bonds represents the uphill phase of a reaction and generates the transition state. The transition state is an unstable high-energy state...
86.6K
Plasma Membrane in Bacteria and Archaea01:27

Plasma Membrane in Bacteria and Archaea

1.7K
The plasma membrane is an essential cellular structure responsible for maintaining cellular integrity and regulating the selective transport of molecules. While bacteria and archaea share the fundamental function of plasma membranes, their structural and molecular differences reflect adaptations to distinct ecological and physiological challenges.Bacterial Plasma MembranesBacterial plasma membranes are predominantly composed of phospholipids with fatty acid chains ester-linked to a glycerol...
1.7K
Drug Distribution: Plasma Protein Binding01:29

Drug Distribution: Plasma Protein Binding

8.8K
Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...
8.8K
Protein Buffers in Blood Plasma and Cells01:20

Protein Buffers in Blood Plasma and Cells

3.8K
The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Fibrinolytic activity and platelet aggregation as risk factors in the progression of diabetic macroarteriopathy of the lower extremities].

Angiologia·1980
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
13:08

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

19.4K

On a Plasminogen Activator from Human Plasma.

A Diaz Batista1, G Hernandez Solana1, J F Corral Almonte1

  • 1The Institute de Hematologia e Immunologia, Habana, Cuba.

Thrombosis and Haemostasis
|February 21, 2019
PubMed
Summary

Researchers purified a plasminogen activator from human plasma kallikrein. This substance exhibits esterase, fibrinolytic, and kininogenase activities, suggesting its potential role in blood clot breakdown.

More Related Videos

Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model
06:20

Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model

Published on: September 1, 2023

1.7K
Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
07:37

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

13.4K

Related Experiment Videos

Last Updated: Jan 29, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
13:08

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

19.4K
Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model
06:20

Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model

Published on: September 1, 2023

1.7K
Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
07:37

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

13.4K

Area of Science:

  • Biochemistry
  • Hematology

Background:

  • Kallikrein is a key enzyme in the kinin-generating system.
  • Plasminogen activators are crucial for fibrinolysis (clot breakdown).

Purpose of the Study:

  • To purify and characterize a plasminogen activating substance from human plasma kallikrein.
  • To investigate the enzymatic activities of the purified substance.

Main Methods:

  • Purification involved QAE-Sephadex A-50 chromatography and Sephadex G-25 gel filtration.
  • Electrophoresis and gel filtration were used for characterization.
  • Enzymatic activities (esterase, fibrinolytic, kininogenase) were quantified.

Main Results:

  • A plasminogen activating substance was successfully purified.
  • The substance has a molecular weight of 15000-18000 daltons and high acid amino acid content.
  • It demonstrated significant esterase, fibrinolytic, and kininogenase activities.

Conclusions:

  • The purified substance is a potent plasminogen activator with multiple enzymatic functions.
  • This activator may play a role in regulating fibrinolysis and kinin generation.