Bioactive poly(ε-caprolactone) microspheres with tunable open pores as microcarriers for tissue regeneration
Anmin Zhou1, Zhaoyang Ye1, Yan Zhou1
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Abstract:
Microparticles with porous structure can be applied as microcarriers for both cell culture and tissue regeneration. While well-controlled pore structure represents a critical challenge to be achieved. In the present study, in order to develop microcarriers for cell culture, a series of poly(ε-caprolactone) microspheres were fabricated with varied macroporous structures. Poly(ε-caprolactone) microspheres were prepared via the integration of the emulsion/solvent evaporation and particle leaching mechanisms. Particularly, by adjusting poly(ε-caprolactone) concentration and the ratio between the porogen paraffin and poly(ε-caprolactone), the microspheres with the pore size of 25.6-84.0 μm and the porosity of 57.4-75.5% were obtained. Further, the microspheres were subjected to alkaline hydrolysis, followed by surface coating with hydroxyapatite. These porous poly(ε-caprolactone) microspheres with surface modification well supported the adhesion and growth of human fibroblasts. Together, bioactive poly(ε-caprolactone) microspheres with controlled pore structure are potential to be applied in cell culture and tissue regeneration.
Related Concept Videos
Neurogenesis and Regeneration of Nervous Tissue
Stem Cell Therapy for Tissue Regeneration
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
Pore Size Distribution
Adequate...
Pore Transport and Ion-Pair Transport
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct...
Whole Body Regeneration
Liver Regeneration
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...


