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Updated: Jan 29, 2026

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
07:31

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Published on: November 1, 2014

12.9K

Species-specificity in the interaction of IgE with FcεRI between humans and rats

Fei Li, Nannan Wang, Yanlei Yang

    Die Pharmazie
    |February 21, 2019
    PubMed

    The interaction of immunoglobulin E (IgE) to its high-affinity receptor (FcεRI) plays a key role in triggering allergic reactions. However, it is still controversial whether species specificity influences the binding ability between humans and rodents. Recombinant hFcεRIα / RBL-2H3 and hFcεRIα / MGC-803 cells were prepared and sensitized with hIgE/anti-hIgE antibody or DNP-IgE/DNP-BSA, respectively. Species-specificity was investigated using immuno-fluorescent analysis, β-hexosaminidase release rate assay, intracellular calcium concentration assay and apoptosis assay, and the results showed that there is species-specificity in IgE/FcεRI in humans and rats, and no cross-recognition between IgE and FcεRI in two species. These results should provide the experimental evidence for further research on the pathogenesis and the drug development of allergic diseases.

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