Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

25.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.1K
Alternative RNA Splicing02:18

Alternative RNA Splicing

5.1K
5.1K
RNA Splicing01:32

RNA Splicing

60.6K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.6K
Null and Alternative Hypotheses01:16

Null and Alternative Hypotheses

12.7K
The actual hypothesis testing begins by considering two hypotheses. They are termed  the null hypothesis and the alternative hypothesis. These hypotheses contain opposing viewpoints.
The null hypothesis, denoted by H0 is a statement of no difference between the variables—they are not related. This can often be considered the status quo. As  a result if you cannot accept the null, it requires some action.
The alternative hypothesis, denoted by H1 or Ha, is a claim about the...
12.7K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.4K
3.4K
Epiphytes, Parasites, and Carnivores02:40

Epiphytes, Parasites, and Carnivores

16.8K
Plants often form mutualistic relationships with soil-dwelling fungi or bacteria to enhance their roots’ nutrient uptake ability. Root-colonizing fungi (e.g., mycorrhizae) increase a plant’s root surface area, which promotes nutrient absorption. While root-colonizing, nitrogen-fixing bacteria (e.g., rhizobia) convert atmospheric nitrogen (N2) into ammonia (NH3), making nitrogen available to plants for various biological functions. For example, nitrogen is essential for the...
16.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GID/CTLH E3 ligase complex control cell fate programs for sexual development of Plasmodium falciparum.

Nature communications·2026
Same author

Potent acridone antimalarial against all three life stages of Plasmodium.

Nature communications·2026
Same author

γδ T cell-derived IL-4 initiates CD8<sup>+</sup> T cell immunity.

Nature immunology·2026
Same author

Novel techniques for disrupting malaria transmission.

Trends in parasitology·2025
Same author

Identifying antimalarials that disrupt malaria parasite transmission when fed to the mosquito.

International journal for parasitology·2025
Same author

Resistance to apicoplast translational inhibitors in Plasmodium.

International journal for parasitology. Drugs and drug resistance·2025
Same journal

<i>Candida</i> spp. suppress neutrophil reactive nitrogen species to evade killing.

mBio·2026
Same journal

Transformation and allelic exchange in <i>Orientia tsutsugamushi</i>.

mBio·2026
Same journal

Limited codiversification of the gut microbiota within humans.

mBio·2026
Same journal

CRISPRi targeting to the <i>Neisseria gonorrhoeae</i> R-loop or G4 mutant region restores pilin antigenic variation.

mBio·2026
Same journal

Dirty mice better recapitulate key features of mRNA vaccine immunogenicity observed in humans.

mBio·2026
Same journal

YdbL directly modulates YdbH-YnbE bridge formation to maintain <i>Escherichia coli</i> outer membrane homeostasis.

mBio·2026
See all related articles

Related Experiment Video

Updated: Jan 28, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.4K

Alternative Splicing in Apicomplexan Parasites.

Lee M Yeoh1,2, V Vern Lee1, Geoffrey I McFadden2

  • 1Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Australia.

Mbio
|February 21, 2019
PubMed
Summary
This summary is machine-generated.

Alternative splicing is crucial for apicomplexan parasites like Plasmodium and Toxoplasma. Disrupting its regulation leads to lethal effects, highlighting its essential role in parasite survival.

Keywords:
PlasmodiumRNA splicingToxoplasmaapicomplexan parasitesposttranscriptional control mechanisms

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.4K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.4K

Related Experiment Videos

Last Updated: Jan 28, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.4K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.4K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.4K

Area of Science:

  • Molecular Biology
  • Parasitology
  • Genomics

Background:

  • Alternative splicing is a key gene regulation mechanism.
  • Apicomplexan parasites utilize alternative splicing for essential functions.
  • The extent of alternative splicing in apicomplexans is debated.

Purpose of the Study:

  • To review methods for accurately assessing alternative splicing in Apicomplexa.
  • To discuss the biological significance of alternative splicing in these parasites.
  • To explore the role of alternative splicing in compact apicomplexan genomes.

Main Methods:

  • Review of recent studies on alternative splicing in apicomplexans.
  • Analysis of transcriptomic data to determine splicing patterns.
  • Investigation of conserved splicing regulators and their disruption.

Main Results:

  • Alternative splicing in apicomplexans can produce noncoding or aberrant transcripts.
  • Regulation of alternative splicing is essential for Plasmodium and Toxoplasma survival.
  • Disruption of splicing regulators causes lethal effects in apicomplexans.

Conclusions:

  • Accurate methods are needed to quantify alternative splicing in Apicomplexa.
  • Alternative splicing plays a vital, conserved role in apicomplexan parasite biology.
  • Understanding alternative splicing is critical for apicomplexan parasite research.