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Dopamine Release Dynamics in the Tuberoinfundibular Dopamine System.

Stefanos Stagkourakis1, Johan Dunevall2, Zahra Taleat3

  • 1Department of Neuroscience, Karolinska Institutet, 17165 Stockholm, Sweden, stefanos.stagkourakis@caltech.edu christian.broberger@ki.se.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|February 21, 2019
PubMed
Summary

Neuroendocrine dopamine neurons control prolactin release. Dopamine release dynamics were studied using optogenetics and voltammetry, revealing frequency-dependent release and transporter function at terminals.

Keywords:
FSCVarcuate nucleusdopamine releasefrequency codinghypothalamustuberoinfundibular

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Area of Science:

  • Neuroscience
  • Neuroendocrinology

Background:

  • Understanding neuronal impulse activity and neurotransmitter release is crucial, especially in the neuroendocrine system.
  • Peptide neurohormone secretion demands high temporal resolution, hindering real-time monitoring.
  • Tuberoinfundibular dopamine (TIDA) neurons control prolactin secretion, offering a unique model for neuroendocrine dopamine dynamics.

Purpose of the Study:

  • To investigate dopamine release dynamics in the male mouse TIDA system.
  • To correlate neuronal discharge frequencies with dopamine output.
  • To explore the role of dopamine transporters and perisomatic release in TIDA neuron regulation.

Main Methods:

  • Combined electrical or optogenetic stimulation of TIDA terminals in the median eminence (ME).
  • Utilized fast-scan cyclic voltammetry for real-time dopamine monitoring.
  • Applied dopamine transporter blocker (methylphenidate) and stimulated TIDA neuron cell bodies.

Main Results:

  • Dopamine output in the ME was maximal at 10 Hz during brief stimulation, mirroring phasic TIDA firing.
  • Maximal dopamine output occurred at 5 Hz during sustained stimulation, matching tonic TIDA firing.
  • Methylphenidate increased ME dopamine levels, confirming transporter function.
  • TIDA neuron stimulation at the cell body induced perisomatic dopamine release.

Conclusions:

  • Neuronal spiking patterns in the neuroendocrine system translate to specific vesicular release dynamics.
  • Dopamine dynamics in the TIDA system are controlled by firing frequency and cellular compartment.
  • Functional dopamine transport exists at neurovascular terminals, and perisomatic release may mediate ultrashort negative feedback.