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Updated: Jan 28, 2026
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A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction
Published on: December 20, 2014
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Reversal Unilateral Ureteral Obstruction: A Mice Experimental Model
Alonso Narváez Barros1,2, Roser Guiteras1, Anna Sola1
1Experimental Nephrology, Department of Ciències Clíniques, Universitat de Barcelona, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona, Spain.
Nephron
|February 21, 2019
Summary
This study describes a reproducible mouse model for reversible unilateral ureteral obstruction (R-UUO) to investigate kidney injury and repair. The model effectively demonstrates acute kidney injury and subsequent functional and histological recovery after obstruction relief.
Area of Science:
- Nephrology
- Renal Pathophysiology
- Animal Models
Background:
- Reversible unilateral ureteral obstruction (R-UUO) serves as a critical model for studying kidney structural and functional recovery post-obstruction.
- This model is particularly valuable for investigating inflammatory, immune, and regenerative processes within the kidney.
Purpose of the Study:
- To establish and describe a reproducible mouse model of renal injury and repair using reversible unilateral ureteral obstruction (R-UUO).
- To evaluate the utility of the R-UUO model in assessing kidney recovery after obstructive uropathy.
Main Methods:
- Eight-week-old male C57BL/6J mice underwent reversible unilateral ureteral obstruction (R-UUO) using a microvascular clamp.
- Kidney function (creatinine), histology, inflammatory markers (F4/80), cytokine expression (IL-2, MCP-1, CD40, TGF-β1), and fibrosis markers (α-smooth muscle actin) were analyzed post-obstruction and after clamp removal.
Main Results:
- The obstructed kidney exhibited acute kidney injury, evidenced by doubled creatinine levels, tubular damage, increased macrophage infiltration, and upregulation of pro-inflammatory/pro-fibrotic cytokines.
- Relief of obstruction led to significant amelioration of kidney function, histological lesions, and inflammatory markers.
Conclusions:
- The R-UUO model provides an easy, reproducible, and reliable method for studying kidney recovery following obstructive uropathy.
- This model facilitates a deeper understanding of the mechanisms underlying kidney repair processes.
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