Effects of paraquat on IL-6 and TNF-α in macrophages

Effects of paraquat (PQ) on interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in macrophages were investigated. Different concentrations of PQ were added to mouse macrophage RAW264.7 for culture. According to different concentrations of PQ, mice were divided into micro concentration (0.01 mmol/l), low concentration (0.1 mmol/l), medium concentration (1 mmol/l), high concentration (10 mmol/l), and control groups without PQ. Trypan blue solution was used for detecting cell viability, a microplate reader for detecting the fluorescence intensity of reactive oxygen species (ROS), ELISA for detecting the expression levels of IL-6 and TNF-α. The medium concentration and the high concentration groups had significantly lower cell viability than the other three groups (P<0.050). The high concentration group had significantly lower cell viability than the medium concentration group (P<0.050). At 1, 4 and 8 h, respectively, the medium and the high concentration groups had significantly higher ROS fluorescence intensity than the other three groups (P<0.050). The high concentration group had significantly higher ROS fluorescence intensity than the medium concentration group (P<0.050). There were significant differences in the expression levels of IL-6 and TNF-α at the 1st, 4th and 8th hour among the five groups (P<0.050). In the micro, the low, the medium and high concentration groups, the expression levels of IL-6 and TNF-α were the lowest at 1 h and the highest at 8 h, which were higher at 4 h than those at 1 h (P<0.050). PQ at a concentration of 1 mmol/l can produce toxicity to macrophages, and greatly increase the ROS fluorescence intensity, the expression levels of IL-6 and TNF-α. PQ poisoning is expected to be treated though IL-6 and TNF-α in the future.