Related Experiment Video

Updated: Jan 28, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.9K

MicroRNA-708-5p affects proliferation and invasion of osteosarcoma cells by targeting URGCP

Cong Sui1, Debao Liu1, Yong Hu1

  • 1Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China.

Experimental and Therapeutic Medicine
|February 21, 2019
PubMed

Insights

MicroRNA-708-5p (miR-708-5p) is downregulated in osteosarcoma. Restoring miR-708-5p levels inhibits cancer cell growth, invasion, and promotes apoptosis, offering potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma is an aggressive skeletal cancer with unclear pathogenesis.
  • Current therapies face challenges due to limited understanding of molecular mechanisms.

Purpose of the Study:

  • Investigate the expression, role, and mechanism of microRNA-708-5p (miR-708-5p) in osteosarcoma development.
  • Determine if miR-708-5p acts as a tumor suppressor in osteosarcoma.

Main Methods:

  • Quantified miR-708-5p expression using reverse transcription-quantitative polymerase chain reaction.
  • Overexpressed miR-708-5p in SaOS-2 osteosarcoma cells.
  • Assessed cell viability, apoptosis, migration, and invasion using standard assays.

Main Results:

  • miR-708-5p was significantly downregulated in osteosarcoma tissues and cells.
  • Overexpression of miR-708-5p inhibited cell viability, invasion, and migration.
  • miR-708-5p overexpression induced apoptosis in SaOS-2 cells and targeted URGCP.

Conclusions:

  • miR-708-5p acts as a tumor suppressor in osteosarcoma.
  • miR-708-5p inhibits osteosarcoma growth and invasion by regulating the URGCP/NF-κB pathway.
  • miR-708-5p represents a potential therapeutic target for osteosarcoma.
Keywords:
metastasismiR-708-5posteosarcomaproliferationup-regulator of cell proliferation

More Related Videos

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.4K
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma09:25

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Published on: October 14, 2016

19.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.0K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.2K
Target Cell Response to Hormones01:22

Target Cell Response to Hormones

Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
5.7K
Mechanical Ventilation II: Invasive Ventilation01:23

Mechanical Ventilation II: Invasive Ventilation

Ventilators are essential medical equipment used to aid patients with respiratory difficulties. Their primary function is to assist or replace spontaneous breathing by providing mechanical ventilation. There are two general classes of mechanical ventilators: negative-pressure and positive-pressure ventilators.
Negative-Pressure Ventilators
Negative-pressure ventilators create a vacuum around the chest or body to draw air into the lungs, simulating breathing. This method does not require an...
744