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Transient active force generation and stress fibre remodelling in cells under cyclic loading
Eoin McEvoy1, Vikram S Deshpande2, Patrick McGarry3
1Discipline of Biomedical Engineering, National University of Ireland Galway, Galway, Ireland.
Biomechanics and Modeling in Mechanobiology
|February 21, 2019
Summary
This study introduces a novel model for actin-myosin cross-bridge cycling in stress fibers (SF). The model predicts cellular force generation and cytoskeletal remodeling under dynamic loading conditions.
Area of Science:
- Cellular mechanics
- Biophysics
- Cytoskeletal dynamics
Background:
- The cytoskeleton is crucial for cell structure and contractility.
- Stress fibers (SF) generate active contractility and remodel under dynamic loads.
- The role of actin-myosin cross-bridge cycling in SF remodeling is not well understood.
Purpose of the Study:
- To develop a novel model for stress fiber (SF) cross-bridge cycling.
- To analyze the influence of transient force generation on cytoskeletal evolution under dynamic loading.
- To predict active cell force generation in response to dynamic loading.
Main Methods:
- Developed a novel SF cross-bridge cycling model.
- Governed cross-bridge formation rates by chemical potentials of myosin heads.
- Coupled the cross-bridge model with a thermodynamic framework for SF remodeling.
- Implemented a 1D model for analysis.
Main Results:
- The model predicts transient active force generation in cells under dynamic loading.
- Successfully predicted complex patterns of active cell force generation.
- Results align with previous experimental findings on cytoskeletal dynamics.
Conclusions:
- The developed model accurately predicts cellular force generation and SF remodeling under dynamic loading.
- Provides new insights into the mechanistic role of actin-myosin cross-bridge cycling.
- The model serves as a valuable tool for studying cytoskeletal mechanics.
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