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Updated: Jan 28, 2026

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons
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Involvement of Actin Filaments in the Cytotoxic Effect of GD2-Specific Antibodies

I I Doronin1,2, I V Kholodenko3, A A Zubareva1

  • 1M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

Bulletin of Experimental Biology and Medicine
|February 21, 2019
PubMed

Insights

GD2-specific antibodies combat neuroblastoma by inducing cell death. This study reveals that actin microfilaments are crucial for this antibody-mediated tumor cell death, highlighting a new therapeutic target.

Area of Science:

  • Oncology
  • Immunology
  • Cell Biology

Background:

  • GD2-specific antibodies are utilized in high-risk neuroblastoma therapy.
  • The precise mechanisms of antibody-triggered cytotoxic signaling in tumor cells are not fully understood.

Purpose of the Study:

  • To investigate the role of cellular components in the cytotoxic effects of GD2-specific antibodies.
  • To elucidate the signaling pathways involved in antibody-dependent cell death of neuroblastoma.

Main Methods:

  • Utilized inhibitor analysis to study the involvement of actin microfilaments.
  • Tested the effect of cytochalasin D on GD2+ tumor cell lines treated with GD2-specific antibodies.

Main Results:

  • Actin microfilaments were identified as key players in antibody-induced cell death.
  • Cytochalasin D significantly antagonized the cytotoxic effect, increasing cell survival by over 20%.
  • A notable decrease in DNA fragmentation was observed in treated cells.

Conclusions:

  • Actin cytoskeleton dynamics are essential for the efficacy of GD2-specific antibody therapy.
  • Targeting actin microfilaments could enhance the antitumor effects of GD2-based immunotherapies for neuroblastoma.
Keywords:
GD2 gangliosideGD2-specific antibodiesactin microfilamentscell deathtumor-associated gangliosides

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