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Updated: Jan 28, 2026

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
Published on: April 27, 2012
In Situ Hybridization for Detection of AAV-Mediated Gene Expression
Jacqueline E Hunter1, Brittney L Gurda1, Sea Young Yoon1,2
1Research Institute of the Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Abstract:
Techniques to localize vector transgenes in cells and tissues are essential in order to fully characterize gene therapy outcomes. In situ hybridization (ISH) uses synthesized complementary RNA or DNA nucleotide probes to localize and detect sequences of interest in fixed cells, tissue sections, or whole tissue mounts. Variations in techniques include adding labels to probes, such as fluorophores, which can allow for the simultaneous visualization of multiple targets. Here we provide the steps necessary to: (1) label probes for colorimetric visualization and (2) perform ISH on OCT cryo-preserved fixed frozen tissues.
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