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Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
Published on: August 11, 2020
Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy
Elena Kirilova1, Ilona Mickevica1, Ligita Mezaraupe2
1Department of Biotechnology, Daugavpils University, Institute of Life Sciences and Technology, Daugavpils, Latvia.
Abstract:
In the present study a new luminescent dye 3-N-(2-pyrrolidinylacetamido)benzanthrone (AZR) was synthesized. Spectroscopic measurements of the novel benzanthrone 3-aminoderivative were performed in seven organic solvents showing strong fluorescence. The capability of the prepared dye for visualization has been tested on flax, red clover and alfalfa to determinate the embryo in plant callus tissue cultures. Callus cells were stained with AZR and further analysed utilizing confocal laser scanning fluorescence microscopy. Performed experiments show high visualization effectiveness of newly synthesized fluorescent dye AZR that is efficient in fast and relatively inexpensive diagnostics of callus embryos that are problematic due to in vitro culture specificity.
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