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Updated: Jan 28, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Insight into bovine (Bos indicus) spermatozoal whole transcriptome profile
Nidhi P Raval1, Tejas M Shah2, Linz-Buoy George1
1Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University, Ahmedabad, 380009, India.
Bovine sperm RNA analysis reveals key genes involved in fertilization and development. This study characterizes the Bos indicus sperm transcriptome, offering insights into reproductive biology.
Area of Science:
- Reproductive Biology
- Genomics
- Molecular Biology
Background:
- Spermatozoa contain coding and non-coding RNAs crucial for reproduction.
- Understanding the bovine sperm transcriptome can elucidate molecular mechanisms in fertility.
Purpose of the Study:
- To analyze the whole transcriptome profile of Bos indicus spermatozoa.
- To identify and characterize global RNA expression in bovine sperm.
Main Methods:
- Whole transcriptome analysis using RNA-Sequencing (RNA-Seq).
- Gene expression quantification using Fragments Per Kilobase Million (FPKM).
- Validation of RNA-Seq data with droplet digital PCR (ddPCR).
Main Results:
- Identified 14,306 genes expressed (FPKM >0) and 405 genes (FPKM >5) in Bos indicus spermatozoa.
- Functional annotation linked sperm transcripts to translation, ribosomal assembly, and sperm functions.
- Validated expression of highly abundant (RN7SL1) and less abundant (ZFP280B) genes.
Conclusions:
- The bovine sperm transcriptome is complex and contains genes vital for reproductive processes.
- This characterization provides a foundation for future research in Bos indicus reproductive biology.
- Identified genes may serve as targets for improving fertility and reproductive efficiency.
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