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Circular RNA expression in exosomes derived from breast cancer cells and patients
Jinyan Wang1, Qian Zhang2, Siying Zhou3
1Department of General Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, PR China.
Aim:
We aimed to explore the roles of circular RNAs (circRNAs) in breast cancer (BCa).
Materials & Methods:
RNA was extracted from exosomes and BCa cells and analyzed using the RNA sequencing technique or microarray.
Results:
Compared with controls, 1147 and 1195 circRNAs were dysregulated in exosomes from metastatic and localized BCa patients, respectively. A total of 480 dysregulated circRNAs were found in metastatic patients compared with localized patients, and these dysregulated circRNAs were enriched in eight pathways. Compared with MCF-7 cells and their exosomes, there were 5842 and 1137 dysregulated circRNAs in MDA-MB-231 cells and exosomes, respectively, and 5 circRNAs were confirmed using real-time quantitative PCR.
Conclusion:
We identified a number of dysregulated circRNAs in exosomes from BCa cells and patients.
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