Related Experiment Video
Updated: Jan 28, 2026

Activating Autophagy by Aerobic Exercise in Mice
Published on: February 3, 2017
[Activation of GPER inhibts proliferation and autophagy in prostatic epithelial cells]
1Department of Urology, the First Affiliated Hospital of Guangzhou Medical College, Guangdong Key Laboratory of Clinical Molecular Medicine and Molecular Diagnosis, Guangzhou 510180, China.
Abstract:
Objective: To investigate the effect of G protein-coupled estrogen receptor (GPER) on proliferation and autophagy in prostatic epithelial cells. Methods: Cell Counting Kit-8 (CCK8) assay was used to observe the growth of BPH-1 and RWPE-1 cells after treating with or not with estradiol or GPER selective agonist G1. Autophagy was quantified with Western blot and Cyto-ID autophagy detection kit after treating with estradiol, G1 or both G1 and G15 in the same cells. Results: The OD value in estrogen group and G1 group was significantly lower than those in control group (P<0.01). Compared with control group, Western blot and Cyto-ID green reagent staining revealed that the ratio of LC3Ⅱ/LC3Ⅰ and the relative fluorescence intensity of BPH-1 and RWPE-1 cells were decreased in G1 group and estradiol group (P<0.01). Pretreatment with G15 reversed the effect of G1 (P<0.05). Conclusion: The activation of GPER leads to the inhibition of autophagy and cell proliferation.
More Related Videos
Related Concept Videos
Inhibition of Cdk Activity
Feedback Inhibition
Autophagy
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Cells Coordinate Growth and Proliferation
Abnormal Proliferation
Enzyme Inhibition

