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Cryopreservation of Synchytrium solstitiale In Planta
1Research Plant Pathologist, European Biological Control Laboratory, United States Department of Agriculture-Agricultural Research Service, Campus International de Baillarguet, CS 90013, Montferrier sur Lez, 34988 St. Gely du Fesc CEDEX, France.
Plant Disease
|February 22, 2019
Summary
Researchers developed a method for long-term storage of Synchytrium solstitiale, a fungus used for biocontrol. Storing infected tissue in 0.5 M sucrose at -2°C allowed active zoospore release after 12 weeks.
Area of Science:
- Plant Pathology
- Mycology
- Biocontrol
Background:
- Synchytrium solstitiale is a potential biocontrol agent for Centaurea solstitialis.
- This obligate parasite requires in planta propagation, necessitating long-term storage methods for research and distribution.
Purpose of the Study:
- To develop a method for preserving Synchytrium solstitiale cultures for extended periods.
- To investigate chemical and cryoprotective treatments affecting zoospore release and viability.
Main Methods:
- Infected Centaurea solstitialis tissue was treated with various chemicals (fungicides, cryoprotectants) and stored at different temperatures.
- Zoospore release and motility were assessed after storage under different conditions.
Main Results:
- Cycloheximide and benomyl inhibited zoospore release or induced encystment.
- Iprodione and propionic acid allowed zoospore release upon transfer to water, but zoospores were initially non-motile.
- Storage at -2°C in 0.5 M sucrose yielded the best results, with active zoospore release after 12 weeks.
Conclusions:
- A viable technique for long-term storage of Synchytrium solstitiale was demonstrated.
- Sucrose at -2°C provides effective cryoprotection for maintaining zoospore viability.
