Related Experiment Video
Updated: Jan 28, 2026

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
Published on: July 27, 2022
Size effect of nano-hydroxyapatite on proliferation of odontoblast-like MDPC-23 cells
1Department of Oral Medicine, School and Hospital of Stomatology, Wenzhou Medical University.
Abstract:
Nano-hydroxyapatite (nano-HAP) is supposed to be a promising candidate for apatite substitute in hard tissue engineering. We aimed to investigate the effect of nano-HAP particles on the proliferation of odontoblast-like MDPC-23 cells compared with conventional hydroxyapatite (c-HAP). HAP in diameter of ~20 nm (np20), ~70 nm (np70) and ~200 nm (c-HAP) were synthesized and characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM). Inverted microscope and MTT assay were used to detect the morphology and proliferation rate of MDPC-23 cells; TEM was used to reveal the internalization of HAP. We found that nano-HAP (np20 and np70), especially np20 expressed obvious growth-promoting effect on MDPC-23 cells compared with c-HAP, which caused the most vacuole in MDPC-23 cells. These results suggest that nano-HAP may be an optimal choice of apatite substitute for MDPC-23 cells on the aspect of cell proliferation.
More Related Videos
Related Concept Videos
Cell Size
Surface Area
Cells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding...
Cells Coordinate Growth and Proliferation
Abnormal Proliferation
Genome Size and the Evolution of New Genes
Brick Sizes
Modular bricks are the most common type and are sized to include the mortar joint, which is essential for...
Maximum Size of Aggregate

