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Updated: Jan 28, 2026
10:17
The Tail Suspension Test
Published on: January 28, 2012
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Integrin αVβ3 Function Influences Citalopram Immobility Behavior in the Tail Suspension Test.
Hope Pan1, Michael R Dohn1, Rody Kingston1
1Department of Pharmacology, Nashville, TN, United States.
Frontiers in Neuroscience
|February 22, 2019
Summary
Integrin αvβ3 function impacts selective serotonin reuptake inhibitor (SSRI) effectiveness. Targeting integrin signaling pathways, like FAK and ERK, is critical for SSRI actions in depression models.
Area of Science:
- Neuroscience
- Molecular Biology
- Pharmacology
Background:
- Genetic and expression studies reveal interactions between integrin β3 and serotonin transporter (SERT) genes.
- Integrin β3-containing receptors (αvβ3) physically interact with SERTs, potentially defining the target for selective serotonin reuptake inhibitors (SSRIs).
Purpose of the Study:
- To investigate how integrin αvβ3 function influences behavioral responses to the SSRI citalopram.
- To identify downstream signaling pathways affected by integrin αvβ3 that modulate SSRI response.
Main Methods:
- Utilized genetically modified mice with altered integrin β3 expression (conditional deletion or constitutively active receptor).
- Assessed behavioral response to citalopram using the tail suspension test (TST).
- Performed antibody array analyses on midbrain synaptosomes and pharmacologically targeted FAK and ERK pathways.
Main Results:
- Mice with altered integrin β3 function showed decreased sensitivity to citalopram in the TST.
- FAK and ERK kinase activities were identified as critical for citalopram's effects.
- A complex relationship between integrin αvβ3, SERT-mediated 5-HT uptake, and citalopram efficacy was revealed.
Conclusions:
- Integrin αvβ3 signaling pathways play a significant role in the behavioral response to SSRIs.
- Findings implicate integrin signaling as a potential therapeutic target for modulating SSRI efficacy in depression treatment.
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