MicroRNA-365 suppressed cell proliferation and migration via targeting PAX6 in glioblastoma

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• MicroRNAs (miRNAs) are crucial regulators in tumor development.
• Glioblastoma is an aggressive primary brain tumor with complex molecular underpinnings.

Purpose of the Study:

• To investigate the role of miR-365 in glioblastoma.
• To determine the expression levels and functional impact of miR-365 in glioblastoma.

Main Methods:

• Quantitative real-time PCR was used to assess miR-365 expression in serum and tissue samples.
• Cell proliferation and migration assays were performed to evaluate the functional effects of miR-365.
• Western blotting was employed to analyze the expression of epithelial-mesenchymal transition markers and PAX6.
• Target gene validation was conducted in U87 glioblastoma cells.

Main Results:

• Serum and tissue expression of miR-365 were significantly downregulated in glioblastoma patients compared to healthy controls and adjacent normal tissues, respectively.
• Overexpression of miR-365 inhibited glioblastoma cell proliferation and migration in vitro.
• miR-365 promoted E-cadherin expression while downregulating N-cadherin and Vimentin, indicating suppression of epithelial-to-mesenchymal transition.
• PAX6 was identified as a direct target of miR-365, and its inhibition by miR-365 contributed to the observed anti-tumor effects.

Conclusions:

• miR-365 functions as a tumor suppressor in glioblastoma.
• Downregulation of miR-365 is associated with glioblastoma progression.
• Targeting miR-365 or its pathway may offer a therapeutic strategy for glioblastoma.