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Updated: Jan 28, 2026

Manipulating the Murine Lacrimal Gland
Published on: November 18, 2014
Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells
Helen P Makarenkova1, Robyn Meech2
1Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA. hmakarenk@scripps.edu.
Abstract:
We present a protocol for isolation of putative epithelial progenitor cells from mouse lacrimal gland (LG) by fluorescence-activated cell sorting (FACS). Isolated LG epithelial progenitor cells can be cultured as 3D reaggregates within extracellular matrix gel or plated as a monolayer. 3D cultures could be maintained for several days and then dissociated with trypsin and plated as monolayer cultures, processed for analysis (e.g., mRNA/protein expression) and/or used for transplantations. Our goal is to provide researchers with a method that can be used as is or modified if isolation of other LG epithelial cell types is required.
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