Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

10.2K
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
10.2K
Voltage01:13

Voltage

4.2K
The movement of electrons in a conductor requires some form of energy or work, usually provided by an external force, like a battery. This force is called the electromotive force or voltage. The voltage between two points, referred to as points "a" and "b," in an electric circuit is the energy (or work) needed to move a unit charge from point "a" to point "b," and this relationship is expressed mathematically as
4.2K
Multiple Voltage Sources01:25

Multiple Voltage Sources

1.8K
Generally, a single battery is not enough to power some devices. In such cases, batteries can be combined in two ways: in series or in parallel.
In series, the positive terminal of one battery is connected to the negative terminal of another battery. Hence, the voltage of each battery is added to give the net voltage, which is increased because each battery boosts the electrons that enter it. The same current flows through each battery because they are connected in series.
Batteries are...
1.8K
Voltage Dividers01:14

Voltage Dividers

1.3K
In electrical circuits, resistors can be connected in series, sequentially linked one after the other. In a series configuration, the same current flows through each resistor. Ohm's law is a fundamental principle to understand the behavior of resistors in series. It expresses the voltage across these resistors in terms of the current and resistance.
Kirchhoff's voltage law implies that the sum of the voltages across the resistors in series equals the source voltage. This means that the current...
1.3K
Three-Phase Voltages01:30

Three-Phase Voltages

573
A three-phase generator produces three voltages that are equal in magnitude but have a phase difference of 120 degrees. This identical magnitude and equal phase separated voltages are known as the balanced voltages and help to minimize power loss while ensuring a steady delivery of energy to connected loads. As voltage sources in a three-phase system can be configured in a wye or a delta formation, the loads connected to these systems can also be arranged in either configuration. This...
573
Nodal Analysis with Voltage Sources01:11

Nodal Analysis with Voltage Sources

2.0K
Nodal analysis is a remarkably effective method used in electrical engineering to simplify the analysis of complex circuits, including those with dependent or independent voltage sources. Its strength lies in its systematic approach to breaking down circuits into manageable components, making it easier for engineers to understand and solve.
Consider a circuit that contains four resistors and two voltage sources, as shown in Figure 1. One of these voltage sources is connected between a...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunofluorescence Analysis for Simultaneous Liquid Biopsy of Multiple Hepatocellular Carcinoma Markers Using a Microfluidic Chip.

Analytical chemistry·2026
Same author

Nocturnal Mating Behavior in Tibetan Macaques: The Low-Ranking Male's Path to Mating Success.

American journal of primatology·2026
Same author

Multicolor room temperature phosphorescence in dibenzothiophene derivative-doped elastic binary polymers for multi-step encryption displays.

Materials horizons·2026
Same author

Effect of thickness ratio on uniaxial mechanical behavior of soft-hard composite rock masses: experimental analysis and modeling.

Scientific reports·2025
Same author

[Determination of tetracyclines in animal muscle tissues by ultra performance liquid chromatography- tandem mass spectrometry].

Se pu = Chinese journal of chromatography·2025
Same author

Impaired NLRP3 inflammasome signaling diverts pyroptotic to apoptotic caspase activation in macrophages.

Frontiers in immunology·2025

Related Experiment Video

Updated: Jan 28, 2026

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
09:53

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Published on: December 31, 2009

12.7K

Low-voltage electrical cell lysis using a microfluidic device.

Xiao-Yu Wei1,2, Jin-Hua Li2, Lei Wang2

  • 1Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China.

Biomedical Microdevices
|February 22, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a new low-voltage method for rapid cell lysis using a microfluidic chip. This technique efficiently lyses single cells and micro-volumes, offering a significant advancement for diagnostics and research.

Keywords:
Alternating currentElectrical cell lysisLow voltageMicrofluidics

More Related Videos

Thermal Measurement Techniques in Analytical Microfluidic Devices
08:29

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

10.1K
Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions
07:39

Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions

Published on: November 17, 2021

4.0K

Related Experiment Videos

Last Updated: Jan 28, 2026

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
09:53

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Published on: December 31, 2009

12.7K
Thermal Measurement Techniques in Analytical Microfluidic Devices
08:29

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

10.1K
Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions
07:39

Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions

Published on: November 17, 2021

4.0K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Cell lysis is crucial for biochemical analysis, diagnostics, and drug screening.
  • Existing cell lysis methods have limitations including sample damage, high voltage requirements, and time consumption.

Purpose of the Study:

  • To develop a simple, fast, and efficient low-voltage method for micro-volume and single cell lysis.
  • To create a microfluidic chip for controlled cell lysis.

Main Methods:

  • Established a low-voltage controllable cell lysis method using a microfluidic chip.
  • Applied low-voltage alternating current (16 Vp-p, 10 kHz) for cell lysis.
  • Investigated lysis efficiency by fluorescently labeling cellular components (whole cell, cytoplasm, nucleus).

Main Results:

  • Achieved simple, efficient, and rapid lysis of micro-volume and single cells.
  • Confirmed complete lysis of whole cells using fluorescent labeling.
  • Demonstrated that higher voltage and optimized frequency enhance lysis efficiency.

Conclusions:

  • The developed low-voltage method provides a novel strategy for micro-volume and single cell lysis.
  • This technique is valuable for on-chip real-time diagnostics and point-of-care (POC) applications.
  • Offers an efficient alternative to traditional, potentially damaging cell lysis methods.