Related Experiment Video
Updated: Jan 28, 2026
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
Published on: March 20, 2020
Proteomics characterisation of central nervous system metastasis biomarkers in triple negative breast cancer
Katerin Rojas L1,2, Lucía Trilla-Fuertes3,2, Angelo Gámez-Pozo3,4
1Department of Medical Oncology, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain.
Background:
Breast cancer (BC) is the most frequent tumour in women. Triple negative tumours (TNBC)-which are associated with minor survival rates-lack markers predictive of response to anticancer drugs. Triple negative tumours frequently metastasise to the central nervous system (CNS).
Objective:
The main objective of this study was to study differences in tumour protein expression between patients with CNS metastases and those without this kind of spread, and propose new biomarkers.
Methods:
A retrospective study was performed. Targeted proteomics and statistical analyses were used to identify possible biomarkers.
Results:
Proteins were quantified by a targeted proteomics approach and protein expression data were successfully obtained from 51 triple negative formalin-fixed paraffin-embedded samples. ISG15, THBS1 and AP1M1 were identified as possible biomarkers related with CNS metastasis development.
Conclusions:
Three possible biomarkers associated with CNS metastases in TNBC tumours were identified: ISG15, THBS1 and AP1M1. They may become markers predicting the appearance of CNS infiltration in triple negative BC.
Related Concept Videos
01:25What is a Nervous System?
01:14The Parasympathetic Nervous System
01:25The Sympathetic Nervous System
02:30Metastasis
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
01:25The Central Dogma
01:21Nervous System